| http://purl.uniprot.org/citations/14701806 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14701806 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14701806 | http://www.w3.org/2000/01/rdf-schema#comment | "We identified VTA1 in a screen for mutations that result in altered vacuole morphology. Deletion of VTA1 resulted in delayed trafficking of the lipophilic dye FM4-64 to the vacuole and altered vacuolar morphology when cells were exposed to the dye 5-(and 6)-carboxy-2',7'-dichlorofluorescein diacetate (CDCFDA). Deletion of class E vacuolar protein sorting (VPS) genes, which encode proteins that affect multivesicular body formation, also showed altered vacuolar morphology upon exposure to high concentrations of CDCFDA. These results suggest a VPS defect for Deltavta1 cells. Deletion of VTA1 did not affect growth on raffinose and only mildly affected carboxypeptidase S sorting. Turnover of the surface protein Ste3p, the a-factor receptor, was affected in Deltavta1 cells with the protein accumulating on the vacuolar membrane. Likewise the alpha-factor receptor Ste2p accumulated on the vacuolar membrane in Deltavta1 cells. We demonstrated that many class E VPS deletion strains are hyper-resistant to the cell wall disruption agent calcofluor white. Deletion of VTA1 or VPS60, another putative class E gene, resulted in calcofluor white hypersensitivity. A Vta1p-green fluorescent protein fusion protein transiently associated with a Pep12p-positive compartment. This localization was altered by deletion of many of the class E VPS genes, indicating that Vta1p binds to endosomes in a manner dependent on the assembly of the endosomal sorting complexes required for transport. Membrane-associated Vta1p co-purified with Vps60p, suggesting that Vta1p is a class E Vps protein that interacts with Vps60p on a prevacuolar compartment."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m312669200"xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m312669200"xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Ward D.M."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Ward D.M."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Kaplan J."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Kaplan J."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Huynh D."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Huynh D."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Shiflett S.L."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Shiflett S.L."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Vaughn M.B."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Vaughn M.B."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Simmons J.C."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/author | "Simmons J.C."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/pages | "10982-10990"xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/pages | "10982-10990"xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/title | "Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/14701806 | http://purl.uniprot.org/core/title | "Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae."xsd:string |