| http://purl.uniprot.org/citations/14701878 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14701878 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14701878 | http://www.w3.org/2000/01/rdf-schema#comment | "Human immunodeficiency virus Rev facilitates the cytoplasmic accumulation of viral RNAs that contain a Rev binding site. A human Rev-interacting protein (hRIP) was originally identified based on its ability to interact with the Rev nuclear export signal (NES) in yeast two-hybrid assays. To date, however, the function of hRIP and a role for hRIP in Rev-directed RNA export have remained elusive. Here we ablate hRIP activity with a dominant-negative mutant or RNA interference and analyze Rev function by RNA in situ hybridization. We find, unexpectedly, that in the absence of functional hRIP, Rev-directed RNAs mislocalize and aberrantly accumulate at the nuclear periphery, where hRIP is localized. In contrast, in the absence of Rev or the Rev cofactor CRM1, Rev-directed RNAs remain nuclear. We further show that the RNA mislocalization pattern resulting from loss of hRIP activity is highly specific to Rev function: the intracellular distribution of cellular poly(A)(+) mRNA, nuclear proteins, and, most important, NES-containing proteins, are unaffected. Thus, hRIP is an essential cellular Rev cofactor, which acts at a previously unanticipated step in HIV-1 RNA export: movement of RNAs from the nuclear periphery to the cytoplasm."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1149704"xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1149704"xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Yu Z."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Yu Z."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Sanchez-Velar N."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Sanchez-Velar N."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Udofia E.B."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Udofia E.B."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Zapp M.L."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/author | "Zapp M.L."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/pages | "23-34"xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/pages | "23-34"xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/title | "hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear region."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/title | "hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear region."xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/14701878 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14701878 |
| http://purl.uniprot.org/citations/14701878 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14701878 |