Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14703438http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the expressions of interleukin 6 (IL-6) and Janus kinases (JAKs) in rats with hypoxia induced pulmonary hypertension (HPH).

Methods

Sixty male Wistar rats were randomized into 5 groups with 12 animals in each: a one-week hypoxic group (H(1) group), a two-week hypoxic group (H(2) group), a three-week hypoxic group (H(3) group), a four-week hypoxic group (H(4) group), a normal oxygen group (N group). The rat model of HPH was replicated in normal baric hypoxic cabin. The levels of IL-6 and JAKs mRNA were measured by using reverse transcription-polymerase chain reaction (RT-PCR); The expression of JAKs protein and cell morphologic changes were observed by immunohistochemistry.

Results

(1) The levels of IL-6 mRNA in H(1) (1.67 +/- 0.09), H(2) (2.26 +/- 0.12) and H(3) (1.55 +/-0.11) groups were significantly higher than that in N group (1.20 +/-0.11, all P < 0.01). The levels of JAK1 mRNA in H(1) (2.11 +/-0.09), H(2) (2.85 +/- 0.12) and H(3) (2.36 +/-0.13) groups were significantly higher than that in N group (1.62 +/-0.10, all P < 0.01); The levels of JAK2 mRNA in H(1) (1.41 +/-0.07), H(2) (2.02 +/- 0.13) and H(3) (1.36 +/-0.09) groups were significantly higher than that in N group (1.01 +/-0.09, all P < 0.01); The levels of JAK3 mRNA in H(1) (0.86 +/-0.11), H(2) (1.45 +/- 0.10) and H(3) (0.91 +/-0.13) groups were significantly higher than that in N group (0.55 +/-0.08, all P < 0.01). The levels of TYK2 mRNA in H(1) (1.36 +/-0.10), H(2) (1.76 +/-0.11) groups were significantly higher than that in N group (0.57 +/-0.07, all P < 0.01). The levels of IL-6 and JAKs mRNA in H(2) group were significantly higher than those in H(1) and H(3) groups (all P < 0.01). (2) Histochemical staining of JAK1 and JAK3 in H(3) group showed yellow cytoplasm of alveolar wall cells, bronchial wall cells and small blood wall cells; The active protein contents of JAK1 (5.36 +/- 0.32) and JAK3 (4.88 +/-0.29) were markedly increased as compared with N group (1.52 +/- 0.18, 1.22 +/-0.09, respectively; all P < 0.01) by TIGER image analysis method.

Conclusions

The levels of IL-6, JAKs mRNA and protein can increase in pulmonary tissues of rats with HPH. It suggests that IL-6/JAKs pathway may take part in the pathogenesis of HPH."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/author"Chen Y."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/author"Chen W.Z."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/author"Wang G.S."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/author"Cai W.Q."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/author"Qian G.S."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/author"Mao B.L."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/name"Zhonghua Jie He He Hu Xi Za Zhi"xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/pages"664-667"xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/title"[Changes of interleukin-6 and Janus kinases in rats with hypoxic pulmonary hypertension]."xsd:string
http://purl.uniprot.org/citations/14703438http://purl.uniprot.org/core/volume"26"xsd:string
http://purl.uniprot.org/citations/14703438http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/14703438
http://purl.uniprot.org/citations/14703438http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/14703438
http://purl.uniprot.org/uniprot/#_A0A8I5ZU24-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/#_A0A8I6AJU1-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/#_A0A8I6AM23-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/#_A0A8L2UK66-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/#_G3V9W2-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/#_O35803-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/#_Q5FVF5-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/#_Q63272-mappedCitation-14703438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/Q5FVF5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/14703438
http://purl.uniprot.org/uniprot/A0A8I5ZU24http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/14703438
▶