| http://purl.uniprot.org/citations/14707140 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14707140 | http://www.w3.org/2000/01/rdf-schema#comment | "Gonadotropin-releasing hormone (GnRH)-induced receptor activation has been demonstrated to entrain a wide variety of signaling modalities. Most signaling pathways are concerned with the control of serine, threonine, or tyrosine-protein kinases, however, in the current article we demonstrate that in both a model cell line and in gonadotropes, GnRH additionally mediates the activation of lipid-directed kinases. We have shown that there is a functional connection between protein-tyrosine kinase modulation and lipid kinase activation. In HEK293 cells stably expressing the Type I mammalian GnRH receptor, we employed a proteomic approach to identify novel protein binding partners for GnRH-activated c-Src. Using matrix-assisted laser desorption ionization time-of-flight mass spectrometry we identified a GnRH-induced association between c-Src and the lipid kinase, diacylglycerol kinase-zeta (DGK-zeta). Using reciprocal co-immunoprecipitation we show that there is a significant elevation of the association between catalytically active c-Src with DGK-zeta in both HEK293 cells and murine gonadotrope LbetaT2 cells. Employing lipid kinase assays we have shown that the catalytic activity of DGK-zeta is significantly heightened in both HEK293 and LbetaT2 cells by GnRH. In addition, we demonstrate that the activation of DGK-zeta exerts a functional role in the murine gonadotrope LbetaT2 cell line. Elevated expression of DGK-zeta resulted in a shortening of the time scale of ERK activation in these cells suggesting a potential role of endogenous DGK-zeta in controlling the induction of LHbeta transcription by ERK1/2."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m310784200"xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/author | "Maudsley S."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/author | "Davidson L."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/author | "Barran P."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/author | "Millar R.P."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/author | "Pawson A.J."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/author | "Freestone S.H."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/author | "Lopez de Maturana R."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/pages | "11906-11916"xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/title | "Gonadotropin-releasing hormone-induced activation of diacylglycerol kinase-zeta and its association with active c-src."xsd:string |
| http://purl.uniprot.org/citations/14707140 | http://purl.uniprot.org/core/volume | "279"xsd:string |
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