| http://purl.uniprot.org/citations/14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14722080 | http://www.w3.org/2000/01/rdf-schema#comment | "The molecular mechanisms required for inositol 1,4,5-trisphosphate receptor (InsP(3)R) targeting to specialized endoplasmic reticulum membrane domains are unknown. We report here a direct, high affinity interaction between InsP(3)R and ankyrin-B and demonstrate that this association is critical for InsP(3)R post-translational stability and localization in cultures of neonatal cardiomyocytes. Recombinant ankyrin-B membrane-binding domain directly interacts with purified cerebellar InsP(3)R (K(d) = 2 nm). 220-kDa ankyrin-B co-immunoprecipitates with InsP(3)R in tissue extracts from brain, heart, and lung. Alanine-scanning mutagenesis of the ankyrin-B ANK (ankyrin repeat) repeat beta-hairpin loop tips revealed that consecutive ANK repeat beta-hairpin loop tips (repeats 22-24) are required for InsP(3)R interaction, thus providing the first detailed evidence of how ankyrin polypeptides associate with membrane proteins. Pulse-chase biosynthesis experiments demonstrate that reduction or loss of ankyrin-B in ankyrin-B (+/-) or ankyrin-B (-/-) neonatal cardiomyocytes leads to approximately 3-fold reduction in half-life of newly synthesized InsP(3)R. Furthermore, interactions with ankyrin-B are required for InsP(3)R stability as abnormal InsP(3)R phenotypes, including mis-localization, and reduced half-life in ankyrin-B (+/-) cardiomyocytes can be rescued by green fluorescent protein (GFP)-220-kDa ankyrin-B but not by GFP-220-kDa ankyrin-B mutants, which do not associate with InsP(3)R. These new results provide the first physiological evidence of a molecular partner required for early post-translational stability of InsP(3)R."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m313979200"xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/author | "Bennett V."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/author | "Davis J.Q."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/author | "Davis L.H."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/author | "Michaely P."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/author | "Mohler P.J."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/author | "Hoffman J.A."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/pages | "12980-12987"xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/title | "Inositol 1,4,5-trisphosphate receptor localization and stability in neonatal cardiomyocytes requires interaction with ankyrin-B."xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/14722080 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14722080 |
| http://purl.uniprot.org/citations/14722080 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/14722080 |
| http://purl.uniprot.org/uniprot/P97582#attribution-3E3E81DA06C481B3463C78EF19E7949E | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_Q3UVA1-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_Q8BQN0-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_Q8CB85-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_P11881-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_S4R2T7-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_Q6NV80-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_Q8C7X9-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |
| http://purl.uniprot.org/uniprot/#_Q8C8N0-mappedCitation-14722080 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14722080 |