| http://purl.uniprot.org/citations/14730085 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14730085 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14730085 | http://www.w3.org/2000/01/rdf-schema#comment | "Mitochondria import hundreds of cytosolically synthesized proteins via the mitochondrial protein import apparatus. Expression analysis in various organs of 19 components of the Arabidopsis mitochondrial protein import apparatus encoded by 31 genes showed that although many were present in small multigene families, often only one member was prominently expressed. This was supported by comparison of real-time reverse transcriptase-polymerase chain reaction and microarray experimental data with expressed sequence tag numbers and massive parallel signature sequence data. Mass spectrometric analysis of purified mitochondria identified 17 import components, their mitochondrial sub-compartment, and verified the presence of TIM8, TIM13, TIM17, TIM23, TIM44, TIM50, and METAXIN proteins for the first time, to our knowledge. Mass spectrometry-detected isoforms correlated with the most abundant gene transcript measured by expression data. Treatment of Arabidopsis cell culture with mitochondrial electron transport chain inhibitors rotenone and antimycin A resulted in a significant increase in transcript levels of import components, with a greater increase observed for the minor isoforms. The increase was observed 12 h after treatment, indicating that it was likely a secondary response. Microarray analysis of rotenone-treated cells indicated the up-regulation of gene sets involved in mitochondrial chaperone activity, protein degradation, respiratory chain assembly, and division. The rate of protein import into isolated mitochondria from rotenone-treated cells was halved, even though rotenone had no direct effect on protein import when added to mitochondria isolated from untreated cells. These findings suggest that transcription of import component genes is induced when mitochondrial function is limited and that minor gene isoforms display a greater response than the predominant isoforms."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.103.033910"xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.103.033910"xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Heazlewood J.L."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Heazlewood J.L."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Millar A.H."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Millar A.H."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Whelan J."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Whelan J."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Lister R."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Lister R."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Chew O."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Chew O."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Clifton R."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Clifton R."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Parker K.L."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Parker K.L."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Lee M.N."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/author | "Lee M.N."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/14730085 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |