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http://purl.uniprot.org/citations/14731133http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14731133http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14731133http://www.w3.org/2000/01/rdf-schema#comment"

Background and aim

Interstitial cells of Cajal (ICC) are pacemakers and mediators of neurotransmission in gastroenteric smooth muscles. Interstitial cells of Cajal require cellular signaling via KIT, a receptor tyrosine kinase, for development and maintenance of cellular phenotype. Much of the evidence demonstrating the functions of ICC comes from studies of W/W V mutant mice, which have reduced KIT function. The aim of the present study was to differentially examine gene expression in the small intestines of wild-type and W/W V mice.

Methods

RNA from the jejunum of wild-type and W/W V mice was analyzed using a differential gene display method.

Results

One candidate gene, encoding a novel small GTPase of the RAS superfamily, was significantly suppressed both in fed and starved W/WV mice. The full-length clone of the murine gene and its human and xenopus counterparts were designated GTP-binding protein, 28 kDa (G28K). Human G28K cDNA encodes a protein of 258 amino acids with homology to the human cell division cycle 42/G25K protein. This gene is located at 1q42.11-q42.3. G28K was abundantly expressed in the human stomach and the small intestine. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed expression of G28K mRNA within single isolated ICC.

Conclusions

Gene analysis showed that G28K was differentially expressed in the small intestines of wild-type and W/W V mice. Interstitial cells of Cajal within the small intestine expressed mRNA for G28K. The specific downregulation of G28K in the jejunum of W/W V mice, and high expression in human intestinal tissue suggest that the G28K gene might be associated with ICC function in mice and in humans."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.org/dc/terms/identifier"doi:10.1111/j.1440-1746.2004.03298.x"xsd:string
http://purl.uniprot.org/citations/14731133http://purl.org/dc/terms/identifier"doi:10.1111/j.1440-1746.2004.03298.x"xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Fujino M.A."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Fujino M.A."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Daigo Y."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Daigo Y."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Sanders K.M."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Sanders K.M."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Ward S.M."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Ward S.M."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Caldas C."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Caldas C."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Ponder B.A.J."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Ponder B.A.J."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Takayama I."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/author"Takayama I."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/name"J. Gastroenterol. Hepatol."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/name"J. Gastroenterol. Hepatol."xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/pages"211-217"xsd:string
http://purl.uniprot.org/citations/14731133http://purl.uniprot.org/core/pages"211-217"xsd:string
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