Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/14750947http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14750947http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14750947http://www.w3.org/2000/01/rdf-schema#comment"

Background

In chromosomal DNA replication, DNA primase initiates the synthesis of a dinucleotide on a single-stranded template DNA, and elongates it to form a primer RNA for the replicative DNA polymerase. Although the apo-structure of an archaeal primase has been reported, the mechanism of primer synthesis by the eukaryotic-type primase still remains to be elucidated.

Results

In this study, we present the crystal structure of the eukaryotic-type DNA primase from the hyperthermophilic archaeon (Pyrococcus horikoshii) with the uridine 5'-triphosphate (UTP). In the present primase-UTP complex, the primase binds the triphosphate moiety of the UTP at the active site, which includes Asp95, Asp97, and Asp280, the essential residues for the nucleotidyl transfer reaction.

Conclusion

The nucleotide binding geometry in this complex explains the previous biochemical analyses of the eukaryotic primase. Based on the complex structure, we constructed a model between the DNA primase and a primer/template DNA for the primer synthesis. This model facilitates the comprehension of the reported features of DNA primase."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.org/dc/terms/identifier"doi:10.1111/j.1365-2443.2003.00693.x"xsd:string
http://purl.uniprot.org/citations/14750947http://purl.org/dc/terms/identifier"doi:10.1111/j.1365-2443.2003.00693.x"xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Hanaoka F."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Hanaoka F."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Shirouzu M."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Shirouzu M."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Ito N."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Ito N."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Yokoyama S."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Yokoyama S."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Nureki O."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/author"Nureki O."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/name"Genes Cells"xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/name"Genes Cells"xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/pages"913-923"xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/pages"913-923"xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/title"Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/title"Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis."xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/volume"8"xsd:string
http://purl.uniprot.org/citations/14750947http://purl.uniprot.org/core/volume"8"xsd:string
▶