| http://purl.uniprot.org/citations/14752510 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14752510 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14752510 | http://www.w3.org/2000/01/rdf-schema#comment | "We reported that the endoplasmic reticulum (ER) stress pathway involving CHOP, a member of the C/EBP transcription factor family, plays a key role in nitric oxide (NO)-mediated apoptosis of macrophages and pancreatic beta cells. We also showed that the cytosolic chaperone pair of hsp70 and dj1 (hsp40/hdj-1) or dj2 (HSDJ/hdj-2) prevents NO-mediated apoptosis upstream of cytochrome c release from mitochondria. To analyze roles of the chaperone pair in preventing apoptosis, RAW 264.7 macrophages stably expressing hsp70 and dj1 or dj2 were established. The chaperone pair prevented LPS/IFN-gamma-induced and NO-mediated apoptosis downstream of CHOP induction. hsp70 mutant protein lacking the ATPase domain or the C-terminal EEVD sequence were not effective in preventing CHOP-induced apoptosis. A mutant dj2 lacking the C-terminal prenylation CaaX motif, was also not effective. When wild-type RAW 264.7 cells were treated with LPS/IFN-gamma, NO-mediated apoptosis was induced, and proapoptotic Bcl-2 family protein Bax was translocated from cytosol to mitochondria. This translocation was prevented in cells stably expressing hsp70/dj2, and in CHOP knockout cells. Overexpression of CHOP in wild-type cells also induced translocation of Bax and this translocation was prevented in cells expressing hsp70/dj2. CHOP-induced apoptosis was prevented by Bax knock-down. Coimmunoprecipitation experiments showed that Bax interacts with both hsp70 and dj1/dj2. ATPase domain of hsp70 was necessary for the binding with Bax. These findings indicate that CHOP-induced apoptosis is mediated by translocation of Bax from the cytosol to the mitochondria, and hsp70/dj1 or dj2 chaperone pair prevents apoptosis by interacting with Bax and preventing translocation to the mitochondria."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.cdd.4401369"xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.cdd.4401369"xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Mori M."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Mori M."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Gotoh T."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Gotoh T."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Terada K."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Terada K."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Oyadomari S."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/author | "Oyadomari S."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/name | "Cell Death Differ."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/name | "Cell Death Differ."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/pages | "390-402"xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/pages | "390-402"xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/title | "hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/title | "hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria."xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/14752510 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14752510 |
| http://purl.uniprot.org/citations/14752510 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14752510 |