| http://purl.uniprot.org/citations/14756312 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14756312 | http://www.w3.org/2000/01/rdf-schema#comment | "The maize transposons Activator (Ac) and Dissociation (Ds) are active in many monocots and dicots, including Arabidopsis. We describe a new Ac-derived transposon construct, designated the Ds-loxP T-DNA, which can be used for both insertional and deletional mutagenesis. There are loxP sites in both orientations on both the transposon and the donor site T-DNA and an arrangement of marker genes that permits selection of transposition events, as well as deletions and inversions extending from the donor site to a transposon reinserted on either side of it. We show that Cre-mediated deletions and inversions occur at a high frequency. The tendency of Ac-Ds transposons to reinsert near the donor site can be used to target both insertional and deletional mutagenesis, but efficient exploitation of this property requires a library of mapped marked donor sites distributed in the genome. We have created a population of independent Ds T-DNA transformants and we have mapped an initial set of 75 Ds T-DNA integration sites. We assessed the potential efficiency of targeted mutagenesis by detecting Ds reinsertion events at several loci over a 400 kb interval from each of two donor sites with different Ds T-DNA constructs. The distribution of reinsertion sites is similar around the two tested loci, with roughly 10, 4, and ca. 1% of reinsertions detected within 1-2 kb of sites 10, 100, and 200-400 kb from the donor site, respectively. To facilitate the use of this targeted mutagenesis system. we have constructed a searchable database of the mapped Ds T-DNA integration sites."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.org/dc/terms/identifier | "doi:10.1023/b:plan.0000009271.08420.d9"xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Huang H."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Ma H."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Zhang S."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Fedoroff N.V."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Raina S."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/author | "Dec E."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/name | "Plant Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/pages | "133-150"xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/title | "Resources for targeted insertional and deletional mutagenesis in Arabidopsis."xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://purl.uniprot.org/core/volume | "53"xsd:string |
| http://purl.uniprot.org/citations/14756312 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14756312 |
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