http://purl.uniprot.org/citations/14760701 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/14760701 | http://www.w3.org/2000/01/rdf-schema#comment | "We have identified the initiation factor 4A (eIF4A) in a two-dimensional protein database of Drosophila wing imaginal discs. eIF4A, a member of the DEAD-box family of RNA helicases, forms the active eIF4F complex that in the presence of eIF4B and eIF4H unwinds the secondary structure of the 5'-UTR of mRNAs during translational initiation. Two-dimensional gel electrophoresis and microsequencing allowed us to purify eIF4A, and generate specific polyclonal antibodies. A combination of immunoblotting and labelling with [(35)S]methionine + [(35)S]cysteine revealed the existence of a single eIF4A isoform encoded by a previously reported gene that maps to chromosome 2L at 26A7-9. Expression of this gene yields two mRNA species, generated by alternative splicing in the 3'-untranslated region. The two mRNAs contain the same open reading frame and produce the identical eIF4A protein. No expression was detected of the eIF4A-related gene CG7483. We detected eIF4A protein expression in the wing imaginal discs of several Drosophila species, and in haltere, leg 1, leg 2, leg 3, and eye-antenna imaginal discs of D. melanogaster. Examination of eIF4A in tumor suppressor mutants showed significantly increased (> 50%) expression in the wing imaginal discs of these larvae. We observed ubiquitous expression of eIF4A mRNA and protein during Drosophila embryogenesis. Yeast two-hybrid analysis demonstrated the in vivo interaction of Drosophila eIF4G with the N-terminal third of eIF4A."xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200300555"xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/author | "Vandekerckhove J."xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/author | "Hernandez G."xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/author | "Santaren J.F."xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/author | "Sierra J.M."xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/author | "Lalioti V."xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/pages | "316-326"xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/title | "Identification and characterization of the expression of the translation initiation factor 4A (eIF4A) from Drosophila melanogaster."xsd:string |
http://purl.uniprot.org/citations/14760701 | http://purl.uniprot.org/core/volume | "4"xsd:string |
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