| http://purl.uniprot.org/citations/14760718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14760718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14760718 | http://www.w3.org/2000/01/rdf-schema#comment | "In the last few years mass spectrometry has become the method of choice for characterization of post-translationally modified proteins. Whereas most protein chemical modifications are binary in the sense that only one change can be associated with a given residue, many different oligosaccharides can be attached to a glycosylation site residue. The detailed characterization of glycoproteins in complex biological samples is extremely challenging. However, information on N-glycosylation can be gained at an intermediary level. Here we demonstrate a procedure for mapping N-glycosylation sites in complex mixtures by reducing sample complexity and enriching glycoprotein content. Glycosylated proteins are selected by an initial lectin chromatography step and digested with endoproteinase Lys-C. Glycosylated peptides are then selected from the digest mixture by a second lectin chromatography step. The glycan components are removed with N-glycosidase F and the peptides digested with trypsin before analysis by on-line reversed-phase liquid chromatography mass spectrometry. Using two different lectins, concanavalin A and wheat germ agglutinin, this procedure was applied to human serum and a total of 86 N-glycosylation sites in 77 proteins were identified."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200300556"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200300556"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Bunkenborg J."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Bunkenborg J."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Pilch B.J."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Pilch B.J."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Podtelejnikov A.V."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Podtelejnikov A.V."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Wisniewski J.R."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/author | "Wisniewski J.R."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/pages | "454-465"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/pages | "454-465"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/title | "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/title | "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/14760718 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14760718 |
| http://purl.uniprot.org/citations/14760718 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14760718 |