| http://purl.uniprot.org/citations/14769047 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14769047 | http://www.w3.org/2000/01/rdf-schema#comment | "Tau protein, a neuronal microtubule-associated protein, forms insoluble fibers ("paired helical filaments") in Alzheimer's disease and other tauopathies. Conflicting views on the structure of the fibers have been proposed recently, ranging from mainly alpha-helical structure to mainly beta-sheet, or a mixture of mostly random coil and beta-sheet. We have addressed this issue by studying tau fibers immunopurified from Alzheimer brain tissue by a conformation-specific antibody and comparing them with fibers reassembled from recombinant tau or tau constructs in vitro, using a combination of electron microscopy and spectroscopic methods. Brain-derived fibers and reassembled fibers both exhibit a typical twisted appearance when examined by electron microscopy. The soluble tau protein is a natively unfolded protein dominated by random coil structure, whereas Alzheimer PHFs and reassembled fibers show a shift toward an increase in the level of beta-structure. The results support a model in which the repeat domain of tau (which lies within the core of PHFs) adopts an increasing level of beta-structure during aggregation, whereas the N- and C-terminal domains projecting away from the PHF core are mostly random coil."xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi0357006"xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/author | "Davies P."xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/author | "Mandelkow E."xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/author | "Barghorn S."xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/pages | "1694-1703"xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/title | "Tau paired helical filaments from Alzheimer's disease brain and assembled in vitro are based on beta-structure in the core domain."xsd:string |
| http://purl.uniprot.org/citations/14769047 | http://purl.uniprot.org/core/volume | "43"xsd:string |
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