| http://purl.uniprot.org/citations/1479357 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1479357 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1479357 | http://www.w3.org/2000/01/rdf-schema#comment | "The primary structures of helices A to G of all bacteriorhodopsin (BR)-like retinal proteins identified in newly isolated halobacteria have been determined from the nucleotide sequence of the BR-like protein genes. Using PCR methods, gene fragments encoding the A-to G-helix region of BR-like proteins were directly amplified from the total genomic DNA of the seven new halobacterial strains. Oligonucleotide primers corresponding to highly conserved regions in the helices A to G were designed from the nucleotide sequences of bacterioopsin (bop) and archaeopsin-I (aop-I), and some primers were effective for the amplification of the gene encoding C-to G-helix region of all new BR-like proteins. The primer corresponding to A-helix region was designed either from the nucleotide sequence of bop and aop-I or from the N-terminus amino acid sequence of a BR-like protein. Three new BR-like proteins were identified from the amino acid sequence, which was deduced from the nucleotide sequence of the genes encoding A-to G-helix region of the BR-like proteins. It was found that not only the amino acid sequence, but also the nucleotide sequence of the gene encoding the C- and G-helix region, in which a number of important residues for proton translocation are located, is highly conserved in three new BR-like proteins. Analysis of the primary structures of the A-to G-helix region of new BR-like proteins revealed that one has about 85% homology with aR-I and aR-II, and the rest have about 55% homology with halobium BR, aR-I and aR-II.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.org/dc/terms/identifier | "doi:10.1099/00221287-138-11-2389"xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.org/dc/terms/identifier | "doi:10.1099/00221287-138-11-2389"xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Otomo J."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Otomo J."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Sasabe H."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Sasabe H."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Tomioka H."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Tomioka H."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Urabe Y."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/author | "Urabe Y."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/name | "J. Gen. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/name | "J. Gen. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/pages | "2389-2396"xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/pages | "2389-2396"xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/title | "The primary structures of helices A to G of three new bacteriorhodopsin-like retinal proteins."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/title | "The primary structures of helices A to G of three new bacteriorhodopsin-like retinal proteins."xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/1479357 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1479357 |
| http://purl.uniprot.org/citations/1479357 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1479357 |