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| http://purl.uniprot.org/citations/1480033 | http://www.w3.org/2000/01/rdf-schema#comment | "The zymogens of matrix metalloproteinase 1 (MMP-1: tissue collagenase), MMP-2 (gelatinase/type IV collagenase) and MMP-3 (stromelysin) were purified from the culture medium of human rheumatoid synovial fibroblasts and the mechanisms of activation of each zymogen by proteinases and 4-aminophenylmercuric acetate (APMA) were studied by kinetic and sequence analyses. The treatment of proMMP-1 (M(r) = 52,000) with proteinases or APMA converted the zymogen to M(r) = 43,000, but it exhibited only 14-25% of the maximal activity. Incubation of a partially active MMP-1 with MMP-3 resulted in rapid, full activation by generating the 41,000-M(r) MMP-1 with Phe81 as the NH2-terminus. MMP-3 directly activated proMMP-1 by cleaving the Gln80-Phe81 bond, but this reaction was extremely slow, indicating that the Gln80-Phe81 bond is not readily available to MMP-3 in the native proMMP-1 molecule. ProMMP-2 (M(r) = 72,000) was activated only by APMA, but not by proteinases. The activation by APMA was rapid and generated an active MMP-2 of M(r) 68,000, but the enzymic activity declined rapidly after activation by autolysis. The NH2-terminal sequence analysis of active MMP-2 indicated that the Asn80-Tyr81 bond was cleaved upon APMA treatment. In contrast, proMMP-3 (M(r) = 57,000) was activated by a variety of proteinases with different specificities. The initial attacks of these proteinases are on a stretch of highly charged groups at the position 34-39 in the propeptide.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/author | "Suzuki K."xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/author | "Salvesen G."xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/author | "Enghild J.J."xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/author | "Nagase H."xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/author | "Morodomi T."xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/name | "Matrix Suppl"xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/pages | "237-244"xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/title | "Activation mechanisms of the precursors of matrix metalloproteinases 1, 2 and 3."xsd:string |
| http://purl.uniprot.org/citations/1480033 | http://purl.uniprot.org/core/volume | "1"xsd:string |
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