| http://purl.uniprot.org/citations/14971903 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14971903 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14971903 | http://www.w3.org/2000/01/rdf-schema#comment | "An LC/MS analysis with diagnostic screening for the detection of peptides with posttranslational modifications revealed the presence of novel sulfated peptides within the alpha-conotoxin molecular mass range in Conus anemone crude venom. A functional assay of the extract showed activity at several neuronal nicotinic acetylcholine receptors (nAChRs). Three sulfated alpha-conotoxins (AnIA, AnIB, and AnIC) were identified by LC/MS and assay-directed fractionation and sequenced after purification. The most active of these, alpha-AnIB, was further characterized and used to investigate the influence of posttranslational modifications on affinity. Synthetic AnIB exhibited subnanomolar potency at the rat alpha3beta2 nAChR (IC50 0.3 nM) and was 200-fold less active on the rat alpha7 nAChR (IC50 76 nM). The unsulfated peptide [Tyr16]AnIB showed a 2-fold and 10-fold decrease in activities at alpha3beta2 (IC50 0.6 nM) and alpha7 (IC50 836 nM) nAChR, respectively. Likewise, removal of the C-terminal amide had a greater influence on potency at the alpha7 (IC50 367 nM) than at the alpha3beta2 nAChR (IC50 0.5 nM). Stepwise removal of two N-terminal glycine residues revealed that these residues affect the binding kinetics of the peptide. Comparison with similar 4/7-alpha-conotoxin sequences suggests that residue 11 (alanine or glycine) and residue 14 (glutamine) constitute important determinants for alpha3beta2 selectivity, whereas the C-terminal amidation and sulfation at tyrosine-16 favor alpha7 affinity."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.org/dc/terms/identifier | "doi:10.1021/jm031010o"xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.org/dc/terms/identifier | "doi:10.1021/jm031010o"xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Adams D.J."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Adams D.J."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Alewood P.F."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Alewood P.F."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Jones A."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Jones A."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Lewis R.J."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Lewis R.J."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Nicke A."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Nicke A."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Loughnan M.L."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/author | "Loughnan M.L."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/name | "J. Med. Chem."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/name | "J. Med. Chem."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/pages | "1234-1241"xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/pages | "1234-1241"xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/title | "Chemical and functional identification and characterization of novel sulfated alpha-conotoxins from the cone snail Conus anemone."xsd:string |
| http://purl.uniprot.org/citations/14971903 | http://purl.uniprot.org/core/title | "Chemical and functional identification and characterization of novel sulfated alpha-conotoxins from the cone snail Conus anemone."xsd:string |