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http://purl.uniprot.org/citations/14976389http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14976389http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14976389http://www.w3.org/2000/01/rdf-schema#comment"

Background

Epicoccum purpurascens (formerly nigrum) (EP), is a ubiquitous saprophytic mould found both indoors and outdoors. Several studies have reported sensitization to EP in 5-7% of different populations worldwide. The diagnosis of mould allergy requires a standardized fungal extract that contains all its important allergenic proteins. The crude allergen extract from EP was standardized earlier, however none of its allergens have been purified.

Methods

A major allergen from spore-mycelia extract of EP was purified using concanavalin A (Con A) Sepharose chromatography, gel filtration and electro-elution. The allergen isolated was characterized for its IgE-binding ability and cross-reactivity with five well-known allergenic fungi by ELISA and immunoblot.

Results

A 33.5-kD glycoprotein allergen of EP, Epi p 1, was purified to homogeneity. All the EP allergic patients' sera tested recognized this protein. Periodate modification of Epi p 1 showed partial loss in IgE binding while proteinase K treatment caused complete loss in binding to IgE. Dose-dependent inhibition in binding of rabbit anti Epi p 1 antibodies was obtained with Epi p 1, Aspergillus fumigatus, Alternaria alternata, Curvularia lunata, Cladosporium herbarum and Fusarium solani in ELISA. Rabbit antibodies to all the above five fungi recognized Epi p 1 in immunoblot, confirming that Epi p 1 shares common epitopes with the fungi tested.

Conclusion

A major glycoprotein allergen of 33.5 kD was purified from EP which cross-reacts with other fungi. Hence this glycoprotein can be exploited to reduce the panel of allergen extracts used for therapy of mould allergy."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.org/dc/terms/identifier"doi:10.1159/000076827"xsd:string
http://purl.uniprot.org/citations/14976389http://purl.org/dc/terms/identifier"doi:10.1159/000076827"xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Arora N."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Arora N."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Singh B.P."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Singh B.P."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Sridhara S."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Sridhara S."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Bisht V."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Bisht V."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Gaur S.N."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/author"Gaur S.N."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/name"Int. Arch. Allergy Immunol."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/name"Int. Arch. Allergy Immunol."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/pages"217-224"xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/pages"217-224"xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/title"Purification and characterization of a major cross-reactive allergen from Epicoccum purpurascens."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/title"Purification and characterization of a major cross-reactive allergen from Epicoccum purpurascens."xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/volume"133"xsd:string
http://purl.uniprot.org/citations/14976389http://purl.uniprot.org/core/volume"133"xsd:string
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