| http://purl.uniprot.org/citations/14976552 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14976552 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14976552 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently demonstrated that the LKB1 tumour suppressor kinase, in complex with the pseudokinase STRAD and the scaffolding protein MO25, phosphorylates and activates AMP-activated protein kinase (AMPK). A total of 12 human kinases (NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK) are related to AMPK. Here we demonstrate that LKB1 can phosphorylate the T-loop of all the members of this subfamily, apart from MELK, increasing their activity >50-fold. LKB1 catalytic activity and the presence of MO25 and STRAD are required for activation. Mutation of the T-loop Thr phosphorylated by LKB1 to Ala prevented activation, while mutation to glutamate produced active forms of many of the AMPK-related kinases. Activities of endogenous NUAK2, QIK, QSK, SIK, MARK1, MARK2/3 and MARK4 were markedly reduced in LKB1-deficient cells. Neither LKB1 activity nor that of AMPK-related kinases was stimulated by phenformin or AICAR, which activate AMPK. Our results show that LKB1 functions as a master upstream protein kinase, regulating AMPK-related kinases as well as AMPK. Between them, these kinases may mediate the physiological effects of LKB1, including its tumour suppressor function."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.emboj.7600110"xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.emboj.7600110"xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Alessi D.R."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Alessi D.R."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Toth R."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Toth R."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Morrice N.A."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Morrice N.A."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Hardie D.G."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Hardie D.G."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Hawley S.A."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Hawley S.A."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Boudeau J."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Boudeau J."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Deak M."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Deak M."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Goeransson O."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Goeransson O."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Lizcano J.M."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Lizcano J.M."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Maekelae T.P."xsd:string |
| http://purl.uniprot.org/citations/14976552 | http://purl.uniprot.org/core/author | "Maekelae T.P."xsd:string |