| http://purl.uniprot.org/citations/14981521 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14981521 | http://www.w3.org/2000/01/rdf-schema#comment | "With the availability of complete genome sequence for Drosophila melanogaster, one of the next strategic goals for fly researchers is a complete gene knockout collection. The P-element transposon, the workhorse of D. melanogaster molecular genetics, has a pronounced nonrandom insertion spectrum. It has been estimated that 87% saturation of the approximately 13,500-gene complement of D. melanogaster might require generating and analyzing up to 150,000 insertions. We describe specific improvements to the lepidopteran transposon piggyBac and the P element that enabled us to tag and disrupt genes in D. melanogaster more efficiently. We generated over 29,000 inserts resulting in 53% gene saturation and a more diverse collection of phenotypically stronger insertional alleles. We found that piggyBac has distinct global and local gene-tagging behavior from that of P elements. Notably, piggyBac excisions from the germ line are nearly always precise, piggyBac does not share chromosomal hotspots associated with P and piggyBac is more effective at gene disruption because it lacks the P bias for insertion in 5' regulatory sequences."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.org/dc/terms/identifier | "doi:10.1038/ng1314"xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Chen F."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Smith R.D."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Zhao L."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Ventura R."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Margolis J."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Swimmer C."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Singer M.A."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Erickson C."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Duyk G."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Tan L.R."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Joo D."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Buchholz R."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Chong A."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Fisher W.W."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Kopczynski C."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Thibault S.T."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Woo A."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Winberg M.L."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Miyazaki W.Y."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Milash B."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Cheung L.M."xsd:string |
| http://purl.uniprot.org/citations/14981521 | http://purl.uniprot.org/core/author | "Stevens L.M."xsd:string |