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http://purl.uniprot.org/citations/14990684http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14990684http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14990684http://www.w3.org/2000/01/rdf-schema#comment"In this report, we describe a newly developed method for isolating outer membranes from Synechocystis sp. PCC 6803 cells. The purity of the outer membrane fraction was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. We investigated the protein composition of the outer membrane using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry followed by database identification. Forty-nine proteins were identified corresponding to 29 different gene products. All of the identified proteins have a putative N-terminal signal peptide. About 40% of the proteins identified represent hypothetical proteins with unknown function. Among the proteins identified are a Toc75 homologue, a protein that was initially found in the outer envelope of chloroplasts in pea, as well as TolC, putative porins, and a pilus protein. Other proteins identified include ABC transporters and GumB, which has a suggested function in carbohydrate export. A number of proteases such as HtrA were also found in the outer membrane of Synechocystis sp. PCC 6803."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.org/dc/terms/identifier"doi:10.1074/mcp.m300137-mcp200"xsd:string
http://purl.uniprot.org/citations/14990684http://purl.org/dc/terms/identifier"doi:10.1074/mcp.m300137-mcp200"xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Funk C."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Funk C."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Huang F."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Huang F."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Kieselbach T."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Kieselbach T."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Schroder W.P."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Schroder W.P."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Norling B."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Norling B."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Hedman E."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/author"Hedman E."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/name"Mol. Cell. Proteomics"xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/name"Mol. Cell. Proteomics"xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/pages"586-595"xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/pages"586-595"xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/title"Isolation of outer membrane of Synechocystis sp. PCC 6803 and its proteomic characterization."xsd:string
http://purl.uniprot.org/citations/14990684http://purl.uniprot.org/core/title"Isolation of outer membrane of Synechocystis sp. PCC 6803 and its proteomic characterization."xsd:string