| http://purl.uniprot.org/citations/14993226 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14993226 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14993226 | http://www.w3.org/2000/01/rdf-schema#comment | "The HNK-1 carbohydrate epitope is found on many neural cell adhesion molecules. Its structure is characterized by a terminal sulfated glucuronyl acid. The glucuronyltransferases, GlcAT-P and GlcAT-S, are involved in the biosynthesis of the HNK-1 epitope, GlcAT-P as the major enzyme. We overexpressed and purified the recombinant human GlcAT-P from Escherichia coli. Analysis of its enzymatic activity showed that it catalyzed the transfer reaction for N-acetyllactosamine (Galbeta1-4GlcNAc) but not lacto-N-biose (Galbeta1-3GlcNAc) as an acceptor substrate. Subsequently, we determined the first x-ray crystal structures of human GlcAT-P, in the absence and presence of a donor substrate product UDP, catalytic Mn(2+), and an acceptor substrate analogue N-acetyllactosamine (Galbeta1-4GlcNAc) or an asparagine-linked biantennary nonasaccharide. The asymmetric unit contains two independent molecules. Each molecule is an alpha/beta protein with two regions that constitute the donor and acceptor substrate binding sites. The UDP moiety of donor nucleotide sugar is recognized by conserved amino acid residues including a DXD motif (Asp(195)-Asp(196)-Asp(197)). Other conserved amino acid residues interact with the terminal galactose moiety of the acceptor substrate. In addition, Val(320) and Asn(321), which are located on the C-terminal long loop from a neighboring molecule, and Phe(245) contribute to the interaction with GlcNAc moiety. These three residues play a key role in establishing the acceptor substrate specificity."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m400622200"xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m400622200"xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Shiba T."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Shiba T."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kato R."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kato R."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Ishiguro M."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Ishiguro M."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kawasaki T."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kawasaki T."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Wakatsuki S."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Wakatsuki S."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Oka S."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Oka S."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kajihara Y."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kajihara Y."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kakuda S."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Kakuda S."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Tagawa H."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/author | "Tagawa H."xsd:string |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14993226 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |