| http://purl.uniprot.org/citations/15001570 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15001570 | http://www.w3.org/2000/01/rdf-schema#comment | "To understand the factors controlling expression of the cGMP phosphodiesterase type 6 (PDE6) genes, we have characterized the promoter of the human PDE6A gene that encodes the catalytic alpha-subunit. In vivo DNase I hypersensitivity assays revealed two sites immediately upstream of the PDE6A core promoter region. Transient transfection assay in Y79 cells of constructs containing varying lengths of the promoter region showed a decrease in promoter activity with increasing length. The most active segment contained a 177-bp upstream sequence including apparent Crx and Nrl transcription factor binding sites. Both Crx and Nrl transactivated the PDE6A promoter in HEK293 cells and showed a >100-fold increase when coexpressed. Coexpression of a dominant negative inhibitor of Nrl abolished Nrl transactivation but had no effect on Crx. DNase I footprinting assays identified three potential Crx binding sites within a 55-bp segment beginning 29 bp upstream of the transcription start point. Mutation of two of these sites reduced reporter gene activity by as much as 69%. Gel shifts showed that all three Crx sites required a TAAT sequence for efficient binding. Consistent with a requirement for Crx and Nrl in Pde6a promoter activity, Pde6a mRNA is reduced by 87% in the retina of Crx(-/-) mice and is undetectable in Nrl(-/-) mice at postnatal day 10. These results establish that both Nrl and Crx are required for full transcriptional activity of the PDE6A gene."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m401864200"xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Chen S."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Yao S."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Ren Z."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Swaroop A."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Swain P.K."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Zack D.J."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "White J.B."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Pittler S.J."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/author | "Mears A.J."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/pages | "19800-19807"xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/title | "Functional analysis of the rod photoreceptor cGMP phosphodiesterase alpha-subunit gene promoter: Nrl and Crx are required for full transcriptional activity."xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15001570 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15001570 |
| http://purl.uniprot.org/citations/15001570 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15001570 |
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| http://purl.uniprot.org/uniprot/#_O43186-mappedCitation-15001570 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15001570 |
| http://purl.uniprot.org/uniprot/#_D2CFI4-mappedCitation-15001570 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15001570 |