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http://purl.uniprot.org/citations/15005727http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15005727http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15005727http://www.w3.org/2000/01/rdf-schema#comment"

Background

House dust mites are regarded as important indoor allergens. While the most studies mite allergens are low molecular weight (mw), a high mw Dermatophagoides farinae mite paramyosin (Der f 11) has recently been cloned. We have also cloned a novel high mw Dermatophagoides pteronyssinus (Dp) mite allergen, Der p 11.

Objective

The aim of this study was to isolate and express a cDNA gene coding for a Der p 11 allergen, to compare the sequence of Der p 11 with other antigens and to evaluate the presence of IgE reactivity to the recombinant protein (rDer p 11) in the sera of allergic adult patients.

Methods

The full-length Der p 11 gene was isolated by cDNA library screening, 5'-3' rapid amplification of cDNA ends and PCR. The cDNA gene was expressed as a glutathione-S-transferase fusion protein in Escherichia coli. The allergenicity of rDer p 11 was tested by human IgE immunodot or immunoblot assay in a large panel of 100 allergic patients with bronchial asthma, allergic rhinitis or eczema.

Results

Der p 11 is a 2965 bp cDNA gene with a 2625 bp open reading frame coding for a 875 amino acid protein. The deduced amino acid sequence of the Der p 11 showed significant homology with various invertebrate paramyosins. The prevalence of serum IgE reactivity to rDer p 11 on immunodot assay ranged from 41.7% to 66.7% in different allergic patient groups, whereas it was rare in non-atopic patients with urticaria (18.8%) and in normal individuals (8%). A high frequency (five out of eight) of MAST(Dp)-allergic serum samples had specific IgE-binding activity to rDer p 11 or its fragments on immunoblot assay, even though their IgE-binding activity to Dp extract was either weak or negative.

Conclusion

The 103-kDa Der p 11 appears to be major Dp mite allergen with a high frequency of IgE reactivity in sera of patients allergic to mites."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.org/dc/terms/identifier"doi:10.1111/j.1365-2222.2004.01878.x"xsd:string
http://purl.uniprot.org/citations/15005727http://purl.org/dc/terms/identifier"doi:10.1111/j.1365-2222.2004.01878.x"xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Lin C.Y."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Lin C.Y."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Lee C.S."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Lee C.S."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Han S.H."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Han S.H."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Tsai L.C."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Tsai L.C."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Hung M.W."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Hung M.W."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Chiang Y.T."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Chiang Y.T."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Chao P.L."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Chao P.L."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Chien A.I."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/author"Chien A.I."xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/name"Clin. Exp. Allergy"xsd:string
http://purl.uniprot.org/citations/15005727http://purl.uniprot.org/core/name"Clin Exp Allergy"xsd:string
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