http://purl.uniprot.org/citations/15016356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15016356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15016356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/15016356 | http://www.w3.org/2000/01/rdf-schema#comment | "RumA catalyzes transfer of a methyl group from S-adenosylmethionine (SAM) specifically to uridine 1939 of 23S ribosomal RNA in Escherichia coli to yield 5-methyluridine. We determined the crystal structure of RumA at 1.95 A resolution. The protein is organized into three structural domains: The N-terminal domain contains sequence homology to the conserved TRAM motif and displays a five-stranded beta barrel architecture characteristic of an oligosaccharide/oligonucleotide binding fold. The central domain contains a [Fe(4)S(4)] cluster coordinated by four conserved cysteine residues. The C-terminal domain displays the typical SAM-dependent methyltransferase fold. The catalytic nucleophile Cys389 lies in a motif different from that in DNA 5-methylcytosine methyltransferases. The electrostatic potential surface reveals a predominately positively charged area that covers the concave surface of the first two domains and suggests an RNA binding mode. The iron-sulfur cluster may be involved in the correct folding of the protein or may have a role in RNA binding."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2004.02.009"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2004.02.009"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/author | "Stroud R.M."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/author | "Stroud R.M."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/author | "Agarwalla S."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/author | "Agarwalla S."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/author | "Lee T.T."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/author | "Lee T.T."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/pages | "397-407"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/pages | "397-407"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/title | "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/title | "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase."xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/volume | "12"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://purl.uniprot.org/core/volume | "12"xsd:string |
http://purl.uniprot.org/citations/15016356 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15016356 |
http://purl.uniprot.org/citations/15016356 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15016356 |
http://purl.uniprot.org/citations/15016356 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15016356 |