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http://purl.uniprot.org/citations/15028735http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15028735http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15028735http://www.w3.org/2000/01/rdf-schema#comment"Understanding of Ca(2+) signaling requires the knowledge of proteins involved in this process. Among these proteins are sarco/endoplasmic reticulum Ca(2+)-ATPases (SERCAs) that pump Ca(2+) into the endoplasmic reticulum (ER). Recently, the human SERCA3 gene was shown to give rise to five isoforms (SERCA3a-e (h3a-h3e)). Here we demonstrate the existence of an additional new member, termed SERCA3f (h3f). By reverse transcriptase-PCR using monocytic U937 cell RNA, h3f mRNA was found to exclude the antepenultimate exon 21. h3f mRNA expression appeared as a human-specific splice variant. It was not found in rats or mice. h3f mRNA gave rise to an h3f protein differing in its C terminus from h3a-h3e. Of particular interest, h3f diverged in the first amino acids after the first splice site but presented the same last 21 amino acids as h3b. Consequently, we further investigated the structure-function-location relationships of the h3b and h3f isoforms. Comparative functional study of h3b and h3f recombinant proteins in intact HEK-293 cells and in fractionated membranes showed the following distinct characteristics: (i) resting cytosolic Ca(2+) concentration ([Ca(2+)](c)) and (ii) ER Ca(2+) content ([Ca(2+)](er)); similar characteristics were shown for the following: (i) the effects of the SERCA inhibitor, thapsigargin, on Ca(2+) release ([Ca(2+)](Tg)) and subsequent Ca(2+) entry ([Ca(2+)](e)) and (ii) the low apparent Ca(2+) affinity and the enhanced rate of dephosphorylation of the E(2)P phosphoenzyme intermediate. Subcellular location of h3b and h3f by immunofluorescence and/or confocal microscopy using the h3b- and h3f-specific polyclonal and the pan-h3 monoclonal (PL/IM430) antibodies suggested overlapping but distinct ER location. The endogenous expression of h3f protein was also proved in U937 cells. Altogether these data suggest that the SERCA3 isoforms have a more widespread role in cellular Ca(2+) signaling than previously appreciated."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m314286200"xsd:string
http://purl.uniprot.org/citations/15028735http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m314286200"xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Kovacs T."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Kovacs T."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Andersen J.P."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Andersen J.P."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Bobe R."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Bobe R."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Bredoux R."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Bredoux R."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Clausen J.D."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Clausen J.D."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Corvazier E."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Corvazier E."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Dode L."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Dode L."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Enouf J."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/author"Enouf J."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/15028735http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
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