| http://purl.uniprot.org/citations/15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15033483 | http://www.w3.org/2000/01/rdf-schema#comment | "17beta-Estradiol (E2)-induced rapid functions (from seconds to minutes) can be attributed to a fraction of nuclear estrogen receptor-alpha (ERalpha) localized at the plasma membrane. As a potential mechanism, we postulated that S-palmitoylation of the Cys447 residue may explain the ability of ERalpha to associate to plasma membrane making possible E2-dependent rapid functions [e.g., extracellular regulated kinase (ERK) activation]. Here, we report direct evidence that the mutation of the Cys447 residue to Ala impairs human ERalpha palmitoylation and E2-induced rapid ERK phosphorylation when transfected in ER-devoid HeLa cells. Moreover, the Cys447Ala mutation significantly decreases the E2-induced transactivation of an estrogen responsive element construct probe. Similar effects were obtained treating HeLa cells transfected with wild type ERalpha with the palmitoyl-acyltransferase inhibitor 2-bromo-hexadecanoic acid. Moreover, the deletion of the A-D domains (containing the DNA binding region) of ERalpha had no consequences on [(3)H]palmitate incorporation, whereas no palmitoylation occurred in the ERalpha mutant devoid of the E domain (i.e., ligand binding domain). These results point to the pivotal role of the Cys447 residue in ERalpha palmitoylation and in the modulation of E2-induced non-genomic functions."xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2004.02.129"xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/author | "Visca P."xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/author | "Ascenzi P."xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/author | "Marino M."xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/author | "Fabozzi G."xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/author | "Acconcia F."xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/pages | "878-883"xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/title | "S-palmitoylation modulates human estrogen receptor-alpha functions."xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://purl.uniprot.org/core/volume | "316"xsd:string |
| http://purl.uniprot.org/citations/15033483 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15033483 |
| http://purl.uniprot.org/citations/15033483 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15033483 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW0-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW1-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |
| http://purl.uniprot.org/uniprot/#_Q14268-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |
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| http://purl.uniprot.org/uniprot/#_A0A125SXV9-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW2-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW3-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |
| http://purl.uniprot.org/uniprot/#_B6DU68-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |
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| http://purl.uniprot.org/uniprot/#_A0A125SXV6-mappedCitation-15033483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15033483 |