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http://purl.uniprot.org/citations/15039567http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15039567http://www.w3.org/2000/01/rdf-schema#comment"KaiB is a component of the circadian clock oscillator in cyanobacteria, which are the simplest organisms that exhibit circadian rhythms. KaiB consists of 108 amino-acid residues and has a molecular weight of 12 025 Da. KaiB and Cys-substituted KaiB mutants from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 were expressed as GST-fusion proteins in Escherichia coli, purified and crystallized. The crystals of wild-type KaiB belong to the monoclinic space group P2(1), with unit-cell parameters a = 89.6, b = 71.2, c = 106.8 A, beta = 100.1 degrees. While the native crystals diffract to 3.7 A, osmium derivatives, which show an approximately 4 A shrinkage in the b axis, diffract to 2.6 A. The crystals of the singly Cys-substituted mutant T64C with Hg, which show different morphology, diffract to 2.5 A and belong to the monoclinic space group P2, with unit-cell parameters a = 63.7, b = 33.4, c = 93.7 A, beta = 100.1 degrees. Anomalous difference Patterson maps of the Os- and Hg-derivative crystals had significant peaks in their Harker sections, suggesting that both derivatives are suitable for structure determination."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.org/dc/terms/identifier"doi:10.1107/s0907444904002112"xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/author"Imada K."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/author"Namba K."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/author"Hayashi F."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/author"Ishiura M."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/author"Iwase R."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/author"Uzumaki T."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/name"Acta Crystallogr D Biol Crystallogr"xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/pages"727-729"xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/title"Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1."xsd:string
http://purl.uniprot.org/citations/15039567http://purl.uniprot.org/core/volume"60"xsd:string
http://purl.uniprot.org/citations/15039567http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15039567
http://purl.uniprot.org/citations/15039567http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15039567
http://purl.uniprot.org/uniprot/#_Q79V61-mappedCitation-15039567http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/15039567
http://purl.uniprot.org/uniprot/Q79V61http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/15039567