http://purl.uniprot.org/citations/15040016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15040016 | http://www.w3.org/2000/01/rdf-schema#comment | "AimTo explore the expression of heparanase gene, CD44v6, MMP-7 and nm23 proteins in human gastric carcinoma as well as their relationship with the clinicopathological factors.MethodsReverse transcription polymerase chain reaction (RT-PCR) was used to measure the expressions of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues. Streptavidin-peroxidase (S-P) two step method was used to measure the immunohistochemical expression of CD44v6, MMP-7 and nm23 protein in 43 human gastric carcinomas.ResultsThe expression of heparanase gene was positive in 29 cases of gastric cancer with a positive rate of 67.4%, which was significantly higher than those in adjacent normal gastric tissues (P<0.05). The heparanase mRNA expression was significantly related to advanced stage of disease, serosal infiltration, lymph node metastasis and size of tumors (P<0.05), but not related to tumor location, gross and histological types of the cancer, peritoneal dissemination and liver metastasis (P>0.05). The positive rate of CD44v6, nm23 and MMP-7 proteins was 76.7%, 37.2% and 60.5%, respectively. The CD44v6 protein expression was significantly related to serosal infiltration, lymph node metastasis and TNM stage of disease (P<0.05). The nm23 protein expression was significantly related to the tumor gross appearance, lymph node and peritoneal metastasis (P<0.05). The MMP-7 protein expression was significantly related to serosal infiltration and TNM stage of disease (P<0.05). In an attempt to measure the association between these agents, we found that the expression of heparanase mRNA had significantly negative correlation with the expression of CD44v6 and MMP-7 protein (P<0.05), the expression of MMP-7 protein had significantly positive correlation with the expression of CD44v6 protein (r=0.568, P<0.05), the expression of MMP-7 protein had no correlation with the expression of nm23 protein (P>0.05), and the expression of nm23 protein had no correlation with the expression of CD44v6 protein (P>0.05).ConclusionDespite their different mechanisms of action, heparanase, CD44v6 and nm23 may play important roles in the invasive infiltration and lymph node metastasis in gastric carcinomas. Instead of metastatic spreading, MMP-7 may be just related to the invasion of gastric carcinomas. However, co-detection of these factors may be important to predict metastatic spreading in such patients."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.org/dc/terms/identifier | "doi:10.3748/wjg.v10.i6.776"xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/author | "Wang J.P."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/author | "Chen J.Q."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/author | "Peng J.S."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/author | "He Y.L."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/author | "Cai S.R."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/author | "Ma J.P."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/author | "Zhan W.H."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/name | "World J Gastroenterol"xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/pages | "776-782"xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/title | "Expression of heparanase gene, CD44v6, MMP-7 and nm23 protein and their relationship with the invasion and metastasis of gastric carcinomas."xsd:string |
http://purl.uniprot.org/citations/15040016 | http://purl.uniprot.org/core/volume | "10"xsd:string |
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