| http://purl.uniprot.org/citations/15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15041185 | http://www.w3.org/2000/01/rdf-schema#comment | "Murine acquired immunodeficiency disease (MAIDS) induced by LPBM5 MuLV is characterized by a late-stage lymphoma and hematopoietic cytopenias similar to those observed in human AIDS. The pathogenesis of MAIDS-related lymphoma/cytopenia is unknown but it has been postulated to involve a defective marrow microenvironment or stroma. The basic Fibroblast Growth Factor (bFGF) of stromal origin is an important stimulator for hematopoietic progenitors of several lineages. Long-term bone marrow cultures (LTBMCs) were established and pure stromal cell cultures were used for in vitro infection hematopoietic reconstitution studies. Reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze bFGF gene expression in stromal cells derived from either viral-infected marrow or uninfected marrow. RT-PCR analysis showed a 40% reduction in the expression of bFGF transcript expression from viral-infected stromal cells, however, the levels of bek and flg bFGF receptors remained unchanged indicating virus-infection only inhibited bFGF gene expression in stromal cells. Viral infection was associated with a progressive decrease in bFGF transcript expression 35% of control at day 7, 50% of control at day 14 and 60% of control at day 21 compared to the mock-infected cultures. In addition, for bek and flg the transcript expression in, in vitro-infected primary cultures were comparable to the mock-infected cultures and remained essentially unchanged throughout culture period. Western blot analysis revealed viral-infected stromal cells produced a 45% decrease in bFGF protein production. Reduction of bFGF protein was confirmed by indirect immunofluorescent staining. We report MuLV infection reduces bFGF transcript expression but not its surface-receptors (bek and flg) in infected stromal cells. Impaired hematopoiesis consistently exhibited from MuLV-infected stromal cultures was restored by exogenous bFGF; therefore, bFGF was responsible in restoration of normal marrow stromal support function. These results suggest a role for bFGF deficiency in the pathogenesis of MAIDS-related marrow failure."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2004.01.007"xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/author | "Tse K.F."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/author | "Morrow J.K."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/author | "Hughes N.K."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/author | "Gallicchio V.S."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/author | "Inayat M.S."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/author | "DellaPuca R."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/name | "Virus Res"xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/pages | "175-184"xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/title | "Basic fibroblast growth factor (bFGF) and its receptor expression (bek and flg) In bone marrow stroma of murine AIDS."xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://purl.uniprot.org/core/volume | "101"xsd:string |
| http://purl.uniprot.org/citations/15041185 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15041185 |
| http://purl.uniprot.org/citations/15041185 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15041185 |
| http://purl.uniprot.org/uniprot/#_E9Q7C7-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYM3-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYM4-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYM5-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYM6-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYM7-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYN9-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYP0-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |
| http://purl.uniprot.org/uniprot/#_A1YYP1-mappedCitation-15041185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15041185 |