| http://purl.uniprot.org/citations/15051725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15051725 | http://www.w3.org/2000/01/rdf-schema#comment | "During neurodevelopment neurons increase phospholipid synthesis to generate additional plasma membrane that makes up the growing neurites. Compared with most cell types, neurons contain a high percentage of the polyunsaturated fatty acids (PUFAs) arachidonic acid (AA) and docosahexaenoic acid (DHA). By utilizing PC12 cell lines as a model neuronal cell line, we examined the internalization rate of AA, DHA, and non-essential oleic acid (OA), as well as their effects on neurite outgrowth. When wild type cells were differentiated, the rate of AA and DHA internalization increased 50% more than the rate of OA internalization. When media were supplemented with AA or DHA, the average neurite length was increased by approximately 40%, but supplementation with the same amount of OA had no effect. We also increased the levels of acyl-CoA synthetase-1 (ACS1) and ACS2 proteins to determine whether they contribute to PUFA internalization or neurite outgrowth. Overexpression of ACS1 increased the rate of OA internalization by 55%, and AA and DHA uptake was increased by 25%, but there was no significant change in neurite outgrowth. In ACS2-overexpressing cells, in contrast, the rate of OA internalization increased by 90%, AA by 115%, and DHA by 70%. The average aggregate neurite length in ACS2-overexpressing cells was increased by approximately 40% when the media were supplemented with PUFAs, but there was no change with OA supplementation. Taken together, these results support the hypotheses that ACSs are rate-limiting for fatty acid internalization and that ACS2 enhances neurite outgrowth by promoting PUFA internalization."xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m313460200"xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/author | "Lodish H.F."xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/author | "Marszalek J.R."xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/author | "Dararutana A."xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/author | "Kitidis C."xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/pages | "23882-23891"xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/title | "Acyl-CoA synthetase 2 overexpression enhances fatty acid internalization and neurite outgrowth."xsd:string |
| http://purl.uniprot.org/citations/15051725 | http://purl.uniprot.org/core/volume | "279"xsd:string |
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