| http://purl.uniprot.org/citations/15060069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15060069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15060069 | http://www.w3.org/2000/01/rdf-schema#comment | "Sodium-calcium exchangers have long been considered inert with respect to monovalent cations such as lithium, choline, and N-methyl-d-glucamine. A key question that has remained unsolved is how despite this, Li(+) catalyzes calcium exchange in mammalian tissues. Here we report that a Na(+)/Ca(2+) exchanger, NCLX cloned from human cells (known as FLJ22233), is distinct from both known forms of the exchanger, NCX and NCKX in structure and kinetics. Surprisingly, NCLX catalyzes active Li(+)/Ca(2+) exchange, thereby explaining the exchange of these ions in mammalian tissues. The NCLX protein, detected as both 70- and 55-KDa polypeptides, is highly expressed in rat pancreas, skeletal muscle, and stomach. We demonstrate, moreover, that NCLX is a K(+)-independent exchanger that catalyzes Ca(2+) flux at a rate comparable with NCX1 but without promoting Na(+)/Ba(2+) exchange. The activity of NCLX is strongly inhibited by zinc, although it does not transport this cation. NCLX activity is only partially inhibited by the NCX inhibitor, KB-R7943. Our results provide a cogent explanation for a fundamental question. How can Li(+) promote Ca(2+) exchange whereas the known exchangers are inert to Li(+) ions? Identification of this novel member of the Na(+)/Ca(2+) superfamily, with distinct characteristics, including the ability to transport Li(+), may provide an explanation for this phenomenon."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m401229200"xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m401229200"xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Volokita M."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Volokita M."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Sekler I."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Sekler I."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Hershfinkel M."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Hershfinkel M."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Ohana E."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Ohana E."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Argaman M."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Argaman M."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Beharier O."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Beharier O."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Elgazar V."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Elgazar V."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Palty R."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Palty R."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Silverman W.F."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/author | "Silverman W.F."xsd:string |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15060069 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |