| http://purl.uniprot.org/citations/15067002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15067002 | http://www.w3.org/2000/01/rdf-schema#comment | "The involvement of phospholipase D (PLD) in the regulation of melanogenesis was examined. Treatment of B16 mouse melanoma cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in the activation of PLD and a decrease in melanin content. 1-Butanol, but not 2-butanol, completely blocked the TPA-induced inhibition of melanogenesis, suggesting the involvement of PLD in this event. Reverse transcription-PCR and immunoblot analyses revealed the existence of both PLD isozymes, PLD1 and PLD2, in B16 cells. When PLD1 or PLD2 was introduced into those cells by an adenoviral gene-transfer technique, both PLD1 and PLD2 were activated by TPA. When PLD1 and PLD2 were overexpressed, PLD2 potently caused a decrease in melanin content, whereas the effect of PLD1 expression on melanin content was minimal. Over-expression of PLD2 itself did not affect protein kinase C activity, as assessed by the intracellular distribution and levels of expression of each isoform expressed in B16 cells. The effects of TPA on the down-regulation of basal or alpha-melanocyte-stimulating hormone-enhanced melanogenesis were almost completely blocked by expressing a lipase activity-negative mutant, LN-PLD2, but not by LN-PLD1. Further, the PLD2-induced decrease in melanin content was accompanied by a decrease in the amount and activity of tyrosinase, a key enzyme in melanogenesis, whereas the mRNA level of tyrosinase was unchanged by the over-expression of PLD2. Moreover, treatment with proteasome inhibitors completely blocked the PLD2-induced down-regulation of melanogenesis. Taken together, the present results indicate that the TPA-induced down-regulation of melanogenesis is mediated by PLD2 but not by PLD1 through the ubiquitin proteasome-mediated degradation of tyrosinase. This suggests that PLD2 may play an important role in regulating pigmentation in vivo."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m401786200"xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Hearing V.J."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Nakamura S."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Oka M."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Okada T."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Saito N."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Ueyama T."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Nishigori C."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Ichihashi M."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/author | "Kageyama A."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/pages | "27774-27780"xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/title | "Down-regulation of melanogenesis by phospholipase D2 through ubiquitin proteasome-mediated degradation of tyrosinase."xsd:string |
| http://purl.uniprot.org/citations/15067002 | http://purl.uniprot.org/core/volume | "279"xsd:string |
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