| http://purl.uniprot.org/citations/15070399 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15070399 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15070399 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/15070399 | http://www.w3.org/2000/01/rdf-schema#comment | "Extracts of frozen rat liver were found to catalyse the formation of 3H2O from DL-2-hydroxy[2-3H]glutarate. Three peaks of enzyme activities were observed on separation by chromatography on DEAE-Sepharose. The first and second peaks corresponded to an enzyme acting on L-2-hydroxyglutarate and the third peak corresponded to an enzyme acting on D-2-hydroxyglutarate, as indicated by competitive inhibition of the detritiation of the racemic radioactive compound by the unlabelled L- and D-isomers respectively. The enzyme acting on the D-form was further characterized. It was independent of NAD or NADP and it converted D-2-hydroxyglutarate into a-ketoglutarate, transferring electrons to artificial electron acceptors. It also oxidized D-lactate, D-malate and meso-tartrate and was stimulated by Zn2+, Co2+ and Mn2+, but not by Mg2+ or Ca2+. Subcellular fractionation indicated that it was present in the mitochondrial fraction. The enzyme was further purified by chromatography on Blue Trisacryl and phenyl-Sepharose, up to a stage where only a few bands were still visible by SDS/PAGE. Among the four candidate polypeptides that were identified by MS, one corresponded to a predicted mitochondrial protein homologous with FAD-dependent D-lactate dehydrogenase. The corresponding human protein was expressed in HEK-293 cells and it was shown to catalyse the detritiation of DL-2-hydroxy[2-3H]glutarate with similar properties as the purified rat enzyme."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20031933"xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20031933"xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Rider M.H."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Rider M.H."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "van Schaftingen E."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "van Schaftingen E."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Vertommen D."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Vertommen D."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Noel G."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Noel G."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Achouri Y."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Achouri Y."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Veiga-Da-Cunha M."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/author | "Veiga-Da-Cunha M."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/pages | "35-42"xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/pages | "35-42"xsd:string |
| http://purl.uniprot.org/citations/15070399 | http://purl.uniprot.org/core/title | "Identification of a dehydrogenase acting on D-2-hydroxyglutarate."xsd:string |