| http://purl.uniprot.org/citations/15075275 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15075275 | http://www.w3.org/2000/01/rdf-schema#comment | "Ras proteins are guanine nucleotide-binding proteins that are highly conserved among eukaryotes. They are involved in signal transduction pathways and are tightly regulated by two sets of antagonistic proteins: GTPase-activating proteins (GAPs) inhibit Ras proteins, whereas guanine exchange factors activate them. In this work, we describe Tfs1p, the first physiological inhibitor of a Ras GAP, Ira2p, in Saccharomyces cerevisiae. TFS1 is a multicopy suppressor of the cdc25-1 mutation in yeast and corresponds to the so-called Ic CPY cytoplasmic inhibitor. Moreover, Tfs1p belongs to the phosphatidylethanolamine-binding protein (PEBP) family, one member of which is RKIP, a kinase and serine protease inhibitor and a metastasis inhibitor in prostate cancer. In this work, the results of (i) a two-hybrid screen of a yeast genomic library, (ii) glutathione S-transferase pulldown experiments, (iii) multicopy suppressor tests of cdc25-1 mutants, and (iv) stress resistance tests to evaluate the activation level of Ras demonstrate that Tfs1p interacts with and inhibits Ira2p. We further show that the conserved ligand-binding pocket of Tfs1-the hallmark of the PEBP family-is important for its inhibitory activity."xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.org/dc/terms/identifier | "doi:10.1128/ec.3.2.459-470.2004"xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/author | "Jacquet M."xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/author | "Benedetti H."xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/author | "Schoentgen F."xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/author | "Bureaud N."xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/author | "Chautard H."xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/name | "Eukaryot Cell"xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/pages | "459-470"xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/title | "Tfs1p, a member of the PEBP family, inhibits the Ira2p but not the Ira1p Ras GTPase-activating protein in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/15075275 | http://purl.uniprot.org/core/volume | "3"xsd:string |
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