| http://purl.uniprot.org/citations/1508179 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1508179 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1508179 | http://www.w3.org/2000/01/rdf-schema#comment | "B-Raf, a member of the Raf family of serine/threonine kinases, is expressed primarily in the brain and in the nervous system. In this study, the biochemical properties of the B-Raf protein were investigated in nerve growth factor (NGF)-responsive cell lines and in brain tissues. B-Raf was identified by using phosphopeptide mapping analysis and cDNA analysis as a 95-kDa protein which is primarily localized in the cytosol. NGF rapidly stimulated both serine and threonine phosphorylation in vivo and autophosphorylation activity in vitro of the B-Raf protein. In PC12 cells, B-Raf autokinase activity was induced by both differentiation factors and mitogens, with maximal activity observed after 5 min of factor addition. B-Raf kinase activity was also observed following NGF treatment of SH-SY5Y neuroblastoma cells and in adult mouse brain and hippocampus. Induction of B-Raf kinase activity in NGF-treated PC12 cells required expression of kinase-active trk receptors. Exogenous substrates or a peptide containing the autophosphorylation site became phosphorylated when added to immune complex kinase assays and reduced the in vitro autophosphorylation activity of B-Raf, suggesting that in vitro autophosphorylation sites and exogenous substrates compete for active sites of the B-Raf kinase. Finally, the major in vitro autophosphorylation site of B-Raf was identified as threonine 372 in the conserved region 2 domain. A threonine residue is present at similar positions in all three mammalian Raf family members and may represent a regulatory site for these proteins."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.12.9.3733-3742.1992"xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.12.9.3733-3742.1992"xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Copeland T.D."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Copeland T.D."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Rapp U.R."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Rapp U.R."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Kaplan D.R."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Kaplan D.R."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Stephens R.M."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Stephens R.M."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Morrison D.K."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Morrison D.K."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Sithanandam G."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/author | "Sithanandam G."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/pages | "3733-3742"xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/pages | "3733-3742"xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/title | "95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site."xsd:string |
| http://purl.uniprot.org/citations/1508179 | http://purl.uniprot.org/core/title | "95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site."xsd:string |