| http://purl.uniprot.org/citations/15084519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15084519 | http://www.w3.org/2000/01/rdf-schema#comment | "The co-transcription factor and DNA repair enzyme, Redox effector factor-1/apurinic/apyrimidinic endonuclease (Ref-1/Ape), facilitates DNA binding and transcriptional activity of a number of transactivating factors, including those governing hypoxia-induced gene expression HIF-1. It is not known, however, whether Ref-1/Ape is a component of the hypoxic transcriptional complex. Electrophoretic mobility shift assays failed to detect direct DNA binding of Ref-1/Ape to either the HIF-1 or AP1 DNA recognition sequences present in the hypoxic response element of the VEGF gene. However, immunodepletion of Ref-1/Ape from nuclear extract prevented DNA binding of ATF/CREB and HIF-1 to the HIF-1 DNA recognition sequence. DNA affinity-precipitation analyses showed that Ref-1/Ape was part of the multiprotein transcriptional complex forming on a 64-mer sequence encompassing a minimal hypoxic response element. Immunodepletion of Ref-1/Ape prevented probe association with HIF-1, p300, ATF, and CREB. Co-immunoprecipitation experiments indicated that Ref-1/Ape present in nuclear extract interacted with HIF-1 and p300 but not ATF/CREB. However, when Ref-1/Ape was immunoprecipitated from the oligonucleotide probe, both HIF-1 and p300 remained probe-associated while ATF/CREB co-immunoprecipitated. These findings suggest that Ref-1/Ape is a critical component of the hypoxia-inducible transcriptional complex forming on the VEGF gene's hypoxic response element and that the presence of Ref-1/Ape in the complex is required for the apparent high affinity association between HIF-1 and its DNA recognition sequence."xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.03-1160fje"xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/author | "Wilson G.L."xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/author | "Campbell C.C."xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/author | "Gillespie M.N."xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/author | "Ziel K.A."xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/name | "FASEB J"xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/pages | "986-988"xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/title | "Ref-1/Ape is critical for formation of the hypoxia-inducible transcriptional complex on the hypoxic response element of the rat pulmonary artery endothelial cell VEGF gene."xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/15084519 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15084519 |
| http://purl.uniprot.org/citations/15084519 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15084519 |
| http://purl.uniprot.org/uniprot/P43138#attribution-4AE2370807B06E455FB80A4DFC76A33F | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/P43138#attribution-D2308FF16C2C274C3CCD731D6FF98DA8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/Q3KR79#attribution-D2308FF16C2C274C3CCD731D6FF98DA8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/A6KCI3#attribution-D2308FF16C2C274C3CCD731D6FF98DA8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/F7FD47#attribution-D2308FF16C2C274C3CCD731D6FF98DA8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/Q5BK34#attribution-D2308FF16C2C274C3CCD731D6FF98DA8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/#_A0A0G2QC09-mappedCitation-15084519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/#_D4A8P8-mappedCitation-15084519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/#_A0A1I9XG45-mappedCitation-15084519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/#_A0A8I6G641-mappedCitation-15084519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15084519 |
| http://purl.uniprot.org/uniprot/#_A0A8L2Q612-mappedCitation-15084519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15084519 |