| http://purl.uniprot.org/citations/15090548 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15090548 | http://www.w3.org/2000/01/rdf-schema#comment | "CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter. Par-4 (prostate apoptosis response-4) is a leucine zipper protein involved in neuronal degeneration and cholinergic signaling in Alzheimer's disease. We now report that Par-4 is a negative regulator of CHT1 choline uptake activity. Transfection of neural IMR-32 cells with human CHT1 conferred Na(+)-dependent, HC-3-sensitive choline uptake that was effectively inhibited by cotransfection of Par-4. Mapping studies indicated that the C-terminal half of Par-4 was physically involved in interacting with CHT1, and the absence of Par-4.CHT1 complex formation precluded the loss of CHT1-mediated choline uptake induced by Par-4, indicating that Par-4.CHT1 complex formation is essential. Kinetic and cell-surface biotinylation assays showed that Par-4 inhibited CHT1-mediated choline uptake by reducing CHT1 expression in the plasma membrane without significantly altering the affinity of CHT1 for choline or HC-3. These results suggest that Par-4 is directly involved in regulating choline uptake by interacting with CHT1 and by reducing its incorporation on the cell surface."xsd:string |
| http://purl.uniprot.org/citations/15090548 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m401495200"xsd:string |
| http://purl.uniprot.org/citations/15090548 | http://purl.uniprot.org/core/author | "Xie J."xsd:string |
| http://purl.uniprot.org/citations/15090548 | http://purl.uniprot.org/core/author | "Guo Q."xsd:string |
| http://purl.uniprot.org/citations/15090548 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15090548 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15090548 | http://purl.uniprot.org/core/pages | "28266-28275"xsd:string |
| http://purl.uniprot.org/citations/15090548 | http://purl.uniprot.org/core/title | "Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane."xsd:string |
| http://purl.uniprot.org/citations/15090548 | http://purl.uniprot.org/core/volume | "279"xsd:string |
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