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http://purl.uniprot.org/citations/15102474http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15102474http://www.w3.org/2000/01/rdf-schema#comment"

Objective

We hypothesized that thrombopoietin (TPO) exerts its mitogenic effects on erythroid cells, at least in part, via an interaction of TPO with the cells' erythropoietin receptor (EPO-R).

Methods

We used BaF3 cells stably transfected with EPO-R to demonstrate that TPO alone is sufficient to support the long-term growth and proliferation of BaF3/EPO-R cells and to develop a TPO-dependent variant, BaF3/EPO-R(T), which is highly sensitive to and dependent on TPO for its proliferation. Northern analysis and RT-PCR were used to verify that both BaF3/EPO-R and BaF3/EPO-R(T) cells express EPO-R but lack c-mpl, the TPO receptor. To confirm that TPO responsiveness of BaF3/EPO-R(T) is due to TPO's interaction with EPO-R, EPO-R was downregulated by antisense mRNA.

Results

Downregulation of EPO-R in BaF3/EPO-R(T) cells abolishes responsiveness to both EPO and TPO. Viability of EPO-treated transfectants decreased from 95% to 36%, while that of TPO-treated transfectants decreased from 95% to 9% by 48 hours. Nontransfected BaF3/EPO-R(T), and BaF3/EPO-R(T) transfected with vector alone, remained viable and grew in either EPO or TPO.

Conclusion

Our results suggest a functional EPO-R may be necessary and sufficient for TPO to exert its mitogenic effects on erythroid cells."xsd:string
http://purl.uniprot.org/citations/15102474http://purl.org/dc/terms/identifier"doi:10.1016/j.exphem.2003.10.015"xsd:string
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/author"Cui K."xsd:string
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/author"Rouleau C."xsd:string
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/author"Feldman L."xsd:string
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/name"Exp Hematol"xsd:string
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/pages"140-148"xsd:string
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/title"A functional erythropoietin receptor is necessary for the action of thrombopoietin on erythroid cells lacking c-mpl."xsd:string
http://purl.uniprot.org/citations/15102474http://purl.uniprot.org/core/volume"32"xsd:string
http://purl.uniprot.org/citations/15102474http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15102474
http://purl.uniprot.org/citations/15102474http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15102474
http://purl.uniprot.org/uniprot/#_P14753-mappedCitation-15102474http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/15102474
http://purl.uniprot.org/uniprot/#_Q3UTV9-mappedCitation-15102474http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/15102474
http://purl.uniprot.org/uniprot/P14753http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/15102474
http://purl.uniprot.org/uniprot/Q3UTV9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/15102474