| http://purl.uniprot.org/citations/15122898 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15122898 | http://www.w3.org/2000/01/rdf-schema#comment | "We have used EPR spectroscopy, redox potentiometry, and protein crystallography to characterize the [4Fe-4S] cluster (FS0) of the Escherichia coli nitrate reductase A (NarGHI) catalytic subunit (NarG). FS0 is clearly visible in the crystal structure of NarGHI [Bertero, M. G., et al. (2003) Nat. Struct. Biol. 10, 681-687] but has novel coordination comprising one His residue and three Cys residues. At low temperatures (<15 K), reduced NarGHI exhibits a previously unobserved EPR signal comprising peaks at g = 5.023 and g = 5.556. We have assigned these features to a [4Fe-4S](+) cluster with an S = (3)/(2) ground state, with the g = 5.023 and g = 5.556 peaks corresponding to subpopulations exhibiting DeltaS = (1)/(2) and DeltaS = (3)/(2) transitions, respectively. Both peaks exhibit midpoint potentials of approximately -55 mV at pH 8.0 and are eliminated in the EPR spectrum of apomolybdo-NarGHI. The structure of apomolybdo-NarGHI reveals that FS0 is still present but that there is significant conformational disorder in a segment of residues that includes one of the Cys ligands. On the basis of these observations, we have assigned the high-spin EPR features of reduced NarGHI to FS0."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi049938l"xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/author | "Bertero M.G."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/author | "Strynadka N.C."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/author | "Weiner J.H."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/author | "Cammack R."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/author | "Rothery R.A."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/author | "Blasco F."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/author | "Palak M."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/pages | "5324-5333"xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/title | "The catalytic subunit of Escherichia coli nitrate reductase A contains a novel [4Fe-4S] cluster with a high-spin ground state."xsd:string |
| http://purl.uniprot.org/citations/15122898 | http://purl.uniprot.org/core/volume | "43"xsd:string |
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