| http://purl.uniprot.org/citations/15123660 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15123660 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15123660 | http://www.w3.org/2000/01/rdf-schema#comment | "Skp1 is an adaptor-like protein in E3(SCF)-ubiquitin ligases and other multiprotein complexes of the cytoplasm and nucleus. In Dictyostelium, Skp1 is modified by an unusual pentasaccharide containing a Galalpha1-Fuc linkage, whose formation is examined here. A cytosolic extract from Dictyostelium was found to yield, after 2400-fold purification, an activity that could transfer Gal from UDP-Gal to both a Fuc-terminated glycoform of Skp1 and synthetic Fuc conjugates in the presence of Mn(2+) and dithiothreitol. The microsomal fraction was devoid of activity. The linkage formed was Galalpha1,3Fuc based on co-chromatography with only this synthetic isomer conjugate, and sensitivity to alpha1,3/6-galactosidase. Skp1 exhibited an almost 1000-fold lower K(m) and 35-fold higher V(max) compared with a simple alpha-fucoside, but this advantage was abolished by denaturation or alkylation of Cys residues. A comparison of a complete series of synthetic glycosides representing the non-reducing terminal mono-, di-, and trisaccharides of Skp1 revealed, surprisingly, that the disaccharide is most active owing primarily to a V(max) advantage, but still much less active than Skp1 itself because of a K(m) difference. These findings indicate that alpha-GalT1 is a cytoplasmic enzyme whose modification of Skp1 requires proper presentation of the terminal acceptor disaccharide by a folded Skp1 polypeptide, which correlates with previous evidence that the Galalpha1,3Fuc linkage is deficient in expressed mutant Skp1 proteins."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m313858200"xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m313858200"xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Wang F."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Wang F."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Ercan A."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Ercan A."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Fisher S.Z."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Fisher S.Z."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Ketcham C."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Ketcham C."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Locke R.D."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Locke R.D."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Matta K.L."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Matta K.L."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Sirajud-Doulah K."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "Sirajud-Doulah K."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "West C.M."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "West C.M."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "van der Wel H."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/author | "van der Wel H."xsd:string |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15123660 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |