| http://purl.uniprot.org/citations/15126503 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15126503 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15126503 | http://www.w3.org/2000/01/rdf-schema#comment | "DNA topoisomerase (topo) IIalpha, an essential enzyme for cell proliferation, is targeted to a proteasome-dependent degradation pathway when human tumor cells are glucose-starved. Here we show that the topo IIalpha destabilization depends on the newly identified domain, GRDD (glucose-regulated destruction domain), which was mapped to the N-terminal 70-170 amino acid sequence. Indeed, the deletion of GRDD conferred a stable feature on topo IIalpha, whereas the fusion of GRDD rendered green fluorescent protein unstable under glucose starvation conditions. Nuclear localization was a prerequisite for GRDD function, because the inhibition of nuclear translocation resulted in the suppression of GRDD-mediated topo IIalpha degradation. Further, GRDD was identified as an interactive domain for Jab1/CSN5, which promoted the degradation of topo IIalpha in a manner dependent on the MPN (Mpr1p/Prd1p N terminus) domain. Depleting Jab1/CSN5 by antisense oligonucleotide and treating cells with the CSN-associated kinase inhibitor, curcumin, inhibited topo IIalpha degradation induced by glucose starvation. These findings demonstrate that GRDD can act as a stress-activated degron for regulating topo IIalpha stability, possibly through interaction with the MPN domain of Jab1/CSN5."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m401411200"xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m401411200"xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Andoh T."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Andoh T."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Tsuruo T."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Tsuruo T."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Yun J."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Yun J."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Tomida A."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/author | "Tomida A."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/pages | "31296-31303"xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/pages | "31296-31303"xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/title | "Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/title | "Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5."xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15126503 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15126503 |
| http://purl.uniprot.org/citations/15126503 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15126503 |