| http://purl.uniprot.org/citations/15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15131131 | http://www.w3.org/2000/01/rdf-schema#comment | "Mutational studies of T cell receptor (TCR) contact residues on the surface of the human class I major histocompatibility complex (MHC) molecule HLA-A2 have identified a "functional hot spot" that comprises Arg(65) and Lys(66) and is involved in recognition by most peptide-specific HLA-A2-restricted TCRs. Although there is a significant amount of functional data on the effects of mutations at these positions, there is comparatively little biochemical information that could illuminate their mode of action. Here, we have used a combination of fluorescence anisotropy, functional assays, and Biacore binding experiments to examine the effects of mutations at these positions on the peptide-MHC interaction and TCR recognition. The results indicate that mutations at both position 65 and position 66 influence peptide binding by HLA-A2 to various extents. In particular, mutations at position 66 result in significantly increased peptide dissociation rates. However, these effects are independent of their effects on TCR recognition, and the Arg(65)-Lys(66) region thus represents a true "hot spot" for TCR recognition. We also made the observation that in vitro T cell reactivity does not scale with the half-life of the peptide-MHC complex, as is often assumed. Finally, position 66 is implicated in the "dual recognition" of both peptide and TCR, emphasizing the multiple roles of the class I MHC peptide-binding domain."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m403372200"xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Beck J.C."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Baker B.M."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Biddison W.E."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Turner R.V."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Baxter T.K."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Binz A.K."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Davis-Harrison R.L."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/author | "Gagnon S.J."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/pages | "29175-29184"xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/title | "Strategic mutations in the class I major histocompatibility complex HLA-A2 independently affect both peptide binding and T cell receptor recognition."xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15131131 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15131131 |
| http://purl.uniprot.org/citations/15131131 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15131131 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C548-mappedCitation-15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15131131 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C551-mappedCitation-15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15131131 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N3-mappedCitation-15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15131131 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N4-mappedCitation-15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15131131 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N5-mappedCitation-15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15131131 |
| http://purl.uniprot.org/uniprot/#_A0A076JXB7-mappedCitation-15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15131131 |
| http://purl.uniprot.org/uniprot/#_A0A076L0P9-mappedCitation-15131131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15131131 |