| http://purl.uniprot.org/citations/15140193 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15140193 | http://www.w3.org/2000/01/rdf-schema#comment | "The present study aimed to investigate pathways that contribute to uptake and transcytosis of high-density lipoproteins (HDLs) and HDL-associated alpha-tocopherol (alpha TocH) across an in vitro model of the blood-brain barrier (BBB). In primary porcine brain capillary endothelial cells HDL-associated alpha TocH was taken up in 10-fold excess of HDL holoparticles, indicating efficient selective uptake, a pathway mediated by scavenger receptor class B, type I (SR-BI). SR-BI was present in caveolae of brain capillary endothelial cells and expressed almost exclusively at the apical membrane. Disruption of caveolae with methyl-beta-cyclodextrin (CDX) resulted in (mis)sorting of SR-BI to the basolateral membrane. Immunohistochemistry of porcine brain cryosections revealed SR-BI expression on brain capillary endothelial cells and presumably astrocytic endfeet. HDL-associated [(14)C]alpha TocH taken up by brain capillary endothelial cells was recovered in sucrose gradient fractions containing the majority of cellular caveolin-1, the major caveolae-associated protein. During mass transfer studies using alpha TocH-enriched HDL, approximately 50% of cellular alpha TocH was recovered with the bulk of cellular caveolin-1 and SR-BI. Efflux experiments revealed that a substantial amount of cell-associated [(14)C]alpha TocH could be mobilized into the culture medium. In addition, apical-to-basolateral transport of HDL holoparticles and HDL-associated alpha TocH was saturable. Results from the present study suggest that part of cerebral apolipoprotein A-I and alpha TocH originates from plasma HDL transcytosed across the BBB and that caveolae-located SR-BI facilitates selective uptake of HDL-associated alpha TocH at the BBB."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1471-4159.2004.02373.x"xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/author | "Hammer A."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/author | "Malle E."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/author | "Sattler W."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/author | "Panzenboeck U."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/author | "Balazs Z."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/author | "Quehenberger O."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/author | "Sovic A."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/name | "J Neurochem"xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/pages | "939-950"xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/title | "Uptake and transport of high-density lipoprotein (HDL) and HDL-associated alpha-tocopherol by an in vitro blood-brain barrier model."xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://purl.uniprot.org/core/volume | "89"xsd:string |
| http://purl.uniprot.org/citations/15140193 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15140193 |
| http://purl.uniprot.org/citations/15140193 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15140193 |
| http://purl.uniprot.org/uniprot/P02647#attribution-8CBA3BC82EC8402394906262119AED8C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15140193 |
| http://purl.uniprot.org/uniprot/Q6RVA9#attribution-298932ADB94E9DC460597632F999D39B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15140193 |
| http://purl.uniprot.org/uniprot/Q28832#attribution-8CBA3BC82EC8402394906262119AED8C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15140193 |
| http://purl.uniprot.org/uniprot/Q8SQC1#attribution-298932ADB94E9DC460597632F999D39B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15140193 |
| http://purl.uniprot.org/uniprot/Q8SQC1#attribution-8CBA3BC82EC8402394906262119AED8C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15140193 |
| http://purl.uniprot.org/uniprot/K9J4V5#attribution-298932ADB94E9DC460597632F999D39B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15140193 |