| http://purl.uniprot.org/citations/15143065 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15143065 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15143065 | http://www.w3.org/2000/01/rdf-schema#comment | "Calcium/calmodulin-dependent protein kinase IV (CaMKIV) is a member of the broad substrate specificity class of Ca(2+)/calmodulin (CaM)-dependent protein kinases and functions as a potent stimulator of Ca(2+)-dependent gene expression. Activation of CaMKIV is a transient, tightly regulated event requiring both Ca(2+)/CaM binding and phosphorylation of the kinase on T200 by an upstream CaMK kinase (CaMKK). Previously, CaMKIV was shown to stably associate with protein serine/threonine phosphatase 2A (PP2A), which was proposed to play a role in negatively regulating the kinase. Here we report that the Ca(2+)/CaM binding-autoinhibitory domain of CaMKIV is required for association of the kinase with PP2A and that binding of PP2A and Ca(2+)/CaM appears to be mutually exclusive. We demonstrate that inhibition of the CaMKIV/PP2A association in cells results in enhanced CaMKIV-mediated gene transcription that is independent of Ca(2+)/CaM. The enhanced transcriptional activity correlates with the elevated level of phospho-T200 that accumulates when CaMKIV is prevented from interacting with PP2A. Collectively, these data suggest a molecular basis for the sequential activation and inactivation of CaMKIV. First, in response to an increase in intracellular Ca(2+), CaMKIV binds Ca(2+)/CaM and becomes phosphorylated on T200 by CaMKK. These events result in the generation of autonomous activity required for CaMKIV-mediated transcriptional regulation. The CaMKIV-associated PP2A then dephosphorylates CaMKIV T200, thereby terminating autonomous activity and CaMKIV-mediated gene transcription."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m404523200"xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m404523200"xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Wadzinski B.E."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Wadzinski B.E."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Anderson K.A."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Anderson K.A."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Means A.R."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Means A.R."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Noeldner P.K."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Noeldner P.K."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Reece K."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/author | "Reece K."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/pages | "31708-31716"xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/pages | "31708-31716"xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/title | "Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/title | "Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex."xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15143065 | http://purl.uniprot.org/core/volume | "279"xsd:string |