| http://purl.uniprot.org/citations/15145941 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15145941 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15145941 | http://www.w3.org/2000/01/rdf-schema#comment | "F-box proteins, components of SCF ubiquitin-ligase complexes, are believed to be responsible for substrate recognition and recruitment in SCF-mediated proteolysis. F-box proteins that have been identified to function in the SCF complexes to date mostly have substrate-binding motifs, such as WD repeats or leucine-rich repeats in their C termini. However, many F-box proteins lack recognizable substrate-binding modules; whether they can function in the SCF complexes remains unclear. We show here that Fbx7, an F-box protein without WD repeats and leucine-rich repeats, is required for the proteasome-mediated proteolysis of the hepatoma up-regulated protein (HURP). Depletion of Fbx7 by small interfering RNA leads to depression of HURP ubiquitination and accumulation of HURP abundance. In the SCF(Fbx7) complex, Fbx7 recruits HURP through its C-terminal proline-rich region in a Cdk1-cyclin B-phosphorylation dependent manner. Mutation of the multiple Cdk1-cyclin B phosphorylation sites on HURP or the proline-rich region of Fbx7 abolishes the association between Fbx7 and HURP. Thus, Fbx7 is a functional adaptor of the SCF complex with a proline-rich region as the substrate-binding module. In addition to Fbx7, data base analyses reveal two putative mammalian proline-rich region-containing F-box proteins, KIAA1783 and RIKEN cDNA 2410015K21. Taken together, these findings further expound the diverse substrate-recognition abilities of the SCF complexes."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m404950200"xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m404950200"xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Hsu J.-M."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Hsu J.-M."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Huang C.-Y.F."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Huang C.-Y.F."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Lee Y.-C.G."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Lee Y.-C.G."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Yu C.-T.R."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/author | "Yu C.-T.R."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/pages | "32592-32602"xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/pages | "32592-32602"xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/title | "Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/title | "Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region."xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15145941 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15145941 |
| http://purl.uniprot.org/citations/15145941 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15145941 |