| http://purl.uniprot.org/citations/15150251 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15150251 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15150251 | http://www.w3.org/2000/01/rdf-schema#comment | "Diterpenoids are naturally occurring plant compounds which have pharmaceutical properties. We have sequenced a 10.4-kbp extension of the dit cluster in Pseudomonas abietaniphila BKME-9, containing genes involved in abietane diterpenoid biodegradation. The ditQ gene was found to encode a cytochrome P450 monooxygenase, P450dit, and to be homologous to the tdtD gene of Pseudomonas diterpeniphila A19-6a. Knocking out ditQ had little effect on growth of BKME-9 on abietic acid but severely impaired growth on dehydroabietic acid (DhA) and palustric acid (PaA), increasing doubling times from 3.8 to 15 h on DhA and from 5.6 to 18.5 h on PaA. A xylE transcriptional fusion showed that transcription of ditQ was induced by a range of diterpenoids. Substrate binding assays of P450dit expressed in Escherichia coli revealed that DhA binds to the enzyme and yields a type I binding spectrum with a Kd of 0.4 microM. These results indicate that P450dit is involved in the metabolism of DhA and PaA and are consistent with its putative role in converting DhA to 7-hydroxy-DhA. Finally, an amino acid sequence identity of greater than 72% and conserved gene arrangement support the hypothesis that the dit gene cluster of P. abietaniphila BKME-9 and the tdt cluster of P. diterpeniphila A19-6a contain functional homologues."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.org/dc/terms/identifier | "doi:10.1128/JB.186.11.3631-3639.2004"xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.186.11.3631-3639.2004"xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/author | "Mohn W.W."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/author | "Mohn W.W."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/author | "Smith D.J."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/author | "Smith D.J."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/author | "Martin V.J."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/author | "Martin V.J."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/name | "J Bacteriol"xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/pages | "3631-3639"xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/pages | "3631-3639"xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/title | "A cytochrome P450 involved in the metabolism of abietane diterpenoids by Pseudomonas abietaniphila BKME-9."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/title | "A cytochrome P450 involved in the metabolism of abietane diterpenoids by Pseudomonas abietaniphila BKME-9."xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/volume | "186"xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://purl.uniprot.org/core/volume | "186"xsd:string |
| http://purl.uniprot.org/citations/15150251 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15150251 |
| http://purl.uniprot.org/citations/15150251 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15150251 |
| http://purl.uniprot.org/citations/15150251 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15150251 |
| http://purl.uniprot.org/citations/15150251 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15150251 |