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http://purl.uniprot.org/citations/15153775http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15153775http://www.w3.org/2000/01/rdf-schema#comment"

Background

The alpha(4)beta(1) integrin (VLA-4) supports rolling and firm adhesion of leukocytes to inflamed tissues via ligation of VCAM-1 or fibronectin expressed on the activated endothelium. We tested the hypothesis that VLA-4 mediates leukocyte recruitment and neointimal growth after arterial injury in the atherosclerosis-prone apolipoprotein E (ApoE)-deficient mouse.

Methods

ApoE (-/-) mice fed a Western diet underwent air desiccation injury, and the expression patterns of VLA-4 and VCAM-1 were determined by immunohistochemistry (IHC). To determine the effect of targeted VLA-4 blockade on leukocyte recruitment and neointimal growth, ApoE (-/-) mice received an intraperitoneal injection of a VLA-4 neutralizing monoclonal antibody (PS/2) at the time of injury alone or over a prolonged administration course. Additional mice received an isotype control antibody.

Results

IHC demonstrated a marked increase in VLA-4 expression 7 days following injury. Prolonged administration of PS/2 resulted in a 72% reduction (p < 0.02) in neointimal growth 28 days following injury. IHC revealed a marked 95% reduction in neutrophil recruitment at 7 days and a 48% reduction in macrophage recruitment 28 days following injury with prolonged PS/2 administration.

Conclusions

Prolonged VLA-4 blockade reduces leukocyte recruitment and neointimal growth following air desiccation injury in ApoE (-/-) mice. These findings demonstrate an important role for VLA-4 in the response to arterial injury."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.org/dc/terms/identifier"doi:10.1159/000078646"xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Sanders J.M."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Phillips J.W."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Ley K.F."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Sarembock I.J."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Bennett D.K."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Barringhaus K.G."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Czarnik A.C."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/author"Thatte J.S."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/name"J Vasc Res"xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/pages"252-260"xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/title"Alpha4beta1 integrin (VLA-4) blockade attenuates both early and late leukocyte recruitment and neointimal growth following carotid injury in apolipoprotein E (-/-) mice."xsd:string
http://purl.uniprot.org/citations/15153775http://purl.uniprot.org/core/volume"41"xsd:string
http://purl.uniprot.org/citations/15153775http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15153775
http://purl.uniprot.org/citations/15153775http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15153775
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http://purl.uniprot.org/uniprot/#_Q00651-mappedCitation-15153775http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/15153775
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