| http://purl.uniprot.org/citations/15163504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15163504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15163504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15163504 | http://www.w3.org/2000/01/rdf-schema#comment | "A reverse genetics system for the generation of influenza virus A/PR/8/34 (NIBSC vaccine strain) from plasmid DNA was developed. Upon transfection of eight bidirectional transcription plasmids encoding the gene segments of A/PR/8/34 into 293T cells, virus titers in the supernatant were about 10(4) TCID50/ml. The production of A/PR/8/34 in 293T cells was compared to that of A/WSN/33, for which virus titers in the supernatant were 10(7)-10(8) TCID50/ml. Time-course analysis of virus production indicated that the differences in virus titers were due to reinfection of 293T cells by A/WSN/33 but not A/PR/8/34. Indeed, virus titers of A/PR/8/34 comparable to those of A/WSN/33 were achieved upon addition of trypsin to the culture medium of transfected cells. The production of chimeric viruses revealed that the difference in virus titers between A/PR/8/34 and A/WSN/33 are determined primarily by differences in the surface glycoproteins hemagglutinin and neuraminidase and the polymerase protein PB1. In conclusion, high-titer virus stocks of recombinant influenza A/PR/8/34 virus can be produced as well as virus stocks with much lower titers, but without the requirement of virus amplification through replication."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2004.02.028"xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2004.02.028"xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2004.02.028"xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Osterhaus A.D.M.E."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Osterhaus A.D.M.E."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Bestebroer T.M."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Bestebroer T.M."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Bestebroer T.M."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Fouchier R.A.M."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Fouchier R.A.M."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Rimmelzwaan G.F."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Rimmelzwaan G.F."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Rimmelzwaan G.F."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Spronken M.I.J."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Spronken M.I.J."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "de Wit E."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "de Wit E."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "de Wit E."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Fouchier R.A."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Fouchier R.A."xsd:string |
| http://purl.uniprot.org/citations/15163504 | http://purl.uniprot.org/core/author | "Osterhaus A.D."xsd:string |