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http://purl.uniprot.org/citations/15169890http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15169890http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15169890http://www.w3.org/2000/01/rdf-schema#comment"Although largely involved in innate and adaptive immunity, NF-kappa B plays an important role in vertebrate development. In chicks, the inactivation of the NF-kappa B pathway induces functional alterations of the apical ectodermal ridge, which mediates limb outgrowth. In mice, the complete absence of NF-kappa B activity leads to prenatal death and neural tube defects. Here, we report the cloning and characterization of NF-kappa B/I kappa B proteins in zebra fish. Despite being ubiquitously expressed among the embryonic tissues, NF-kappa B/I kappa B members present distinct patterns of gene expression during the early zebra fish development. Biochemical assays indicate that zebra fish NF-kappa B proteins are able to bind consensus DNA-binding (kappa B) sites and inhibitory I kappa B alpha proteins from mammals. We show that zebra fish I kappa B alphas are degraded in a time-dependent manner after induction of transduced murine embryo fibroblasts (MEFs) and that these proteins are able to rescue NF-kappa B activity in I kappa B alpha(-/-) MEFs. Expression of a dominant-negative form of the murine I kappa B alpha (mI kappa B alpha M), which is able to block NF-kappa B in zebra fish cells, interferes with the notochord differentiation, generating no tail (ntl)-like embryos. This phenotype can be rescued by coinjection of the T-box gene ntl (Brachyury homologue), which is typically required for the formation of posterior mesoderm and axial development, suggesting that ntl lies downstream of NF-kappa B . We further show that ntl and Brachyury promoter regions contain functional kappa B sites and NF-kappa B can directly modulate ntl expression. Our study illustrates the conservation and compatibility of NF-kappa B/I kappa B proteins among vertebrates and the importance of NF-kappa B pathway in mesoderm formation during early embryogenesis."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.org/dc/terms/identifier"doi:10.1128/MCB.24.12.5257-5268.2004"xsd:string
http://purl.uniprot.org/citations/15169890http://purl.org/dc/terms/identifier"doi:10.1128/mcb.24.12.5257-5268.2004"xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Verma I.M."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Verma I.M."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Correa R.G."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Correa R.G."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Ng J.K."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Ng J.K."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Izpisua-Belmonte J.C."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Izpisua-Belmonte J.C."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Tergaonkar V."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Tergaonkar V."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Dubova I."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/author"Dubova I."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/name"Mol. Cell. Biol."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/name"Mol Cell Biol"xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/pages"5257-5268"xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/pages"5257-5268"xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/title"Characterization of NF-kappa B/I kappa B proteins in zebra fish and their involvement in notochord development."xsd:string
http://purl.uniprot.org/citations/15169890http://purl.uniprot.org/core/title"Characterization of NF-kappa B/I kappa B proteins in zebra fish and their involvement in notochord development."xsd:string
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