| http://purl.uniprot.org/citations/15171717 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15171717 | http://www.w3.org/2000/01/rdf-schema#comment | "The neoplastic production of the insulin-like growth factor binding protein (IGFBP)-2 often correlates with tumor malignancy and aggressiveness. Since IGFBP-2 contains an RGD motif in its C-terminus, it was hypothesized that this protein may act independently of IGF on tumor cells through integrins. To investigate this, integrin binding, intracellular signaling and the impact of IGFBP-2 on cell adhesion and proliferation were examined in two tumor cell lines. In tracer displacement studies, up to 30% of the added (125)I-hIGFBP-2 specifically bound to the cells. Bound (125)I-hIGFBP-2 was reversibly displaced by IGFBP-2, IGFBP-1 and RGD-(Gly-Arg-Asp)-containing peptides, but not by IGFBP-3, -4, -5, -6 and RGE-(Gly-Arg-Glu)-containing peptides. Blocking with antibodies directed against different integrins and with fibronectin demonstrated that IGFBP-2 cell surface binding is specific for alpha5beta1-integrin. Incubation of IGFBP-2 with equimolar quantities of IGF-I and IGF-II annihilated RGD-specific binding. IGFBP-2 binding at the cell surface led to dephosphorylation of the focal adhesion-kinase (FAK) of up to 37% (P<0.01), and of the p42/44 MAP-kinases of up to 40% (P<0.01). In addition, IGFBP-2 promoted de-adhesion of the cells dose-dependently by up to 30% (P<0.05), and reduced proliferation by 24% (P<0.01). Since one of the cell lines used does not express a functional IGF-I receptor, these data demonstrate that IGFBP-2 can act in an IGF-independent manner, at least in part by an interaction with alpha5beta1-integrin."xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.org/dc/terms/identifier | "doi:10.1677/jme.0.0320859"xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/author | "Elmlinger M.W."xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/author | "Ranke M.B."xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/author | "Schutt B.S."xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/author | "Langkamp M."xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/author | "Rauschnabel U."xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/name | "J Mol Endocrinol"xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/pages | "859-868"xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/title | "Integrin-mediated action of insulin-like growth factor binding protein-2 in tumor cells."xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/15171717 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15171717 |
| http://purl.uniprot.org/citations/15171717 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15171717 |
| http://purl.uniprot.org/uniprot/#_C9JMY1-mappedCitation-15171717 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15171717 |
| http://purl.uniprot.org/uniprot/#_P18065-mappedCitation-15171717 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15171717 |
| http://purl.uniprot.org/uniprot/#_Q59FF1-mappedCitation-15171717 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15171717 |
| http://purl.uniprot.org/uniprot/C9JMY1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15171717 |
| http://purl.uniprot.org/uniprot/P18065 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15171717 |
| http://purl.uniprot.org/uniprot/Q59FF1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15171717 |